CD33 anticorps (APC)
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- Antigène Voir toutes CD33 Anticorps
- CD33
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Reactivité
- Humain, Non-Human Primate
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD33 est conjugé à/à la APC
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Application
- Flow Cytometry (FACS)
- Specificité
- The mouse monoclonal antibody WM53 reacts with an extracellular epitope of CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells, it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
- Réactivité croisée (Details)
- Human, Non-Human Primates
- Purification
- Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogène
- Human AML cells
- Clone
- WM53
- Isotype
- IgG1
- Top Product
- Discover our top product CD33 Anticorps primaire
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- Indications d'application
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
- Commentaires
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The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- Restrictions
- For Research Use only
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- Reconstitution
- No reconstitution is necessary.
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
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Do not freeze.
Avoid prolonged exposure to light. - Stock
- 4 °C
- Stockage commentaire
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells." dans: Blood, Vol. 105, Issue 3, pp. 1256-64, (2005) (PubMed).
: "Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function." dans: European heart journal, Vol. 26, Issue 12, pp. 1196-204, (2005) (PubMed).
: "Macrophages expressing triggering receptor expressed on myeloid cells-1 are underrepresented in the human intestine." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 1, pp. 517-24, (2004) (PubMed).
: "Surface expression and function of p75/AIRM-1 or CD33 in acute myeloid leukemias: engagement of CD33 induces apoptosis of leukemic cells." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 10, pp. 5764-9, (2001) (PubMed).
: "Expression of leukemia-associated antigen, JL1, in bone marrow and thymus." dans: The American journal of pathology, Vol. 158, Issue 4, pp. 1473-80, (2001) (PubMed).
: "Unusual immunophenotypes in acute leukaemias: incidence and clinical correlations." dans: British journal of haematology, Vol. 72, Issue 4, pp. 512-8, (1989) (PubMed).
: "Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging ..." dans: British journal of haematology, Vol. 69, Issue 2, pp. 163-71, (1988) (PubMed).
: "Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33)." dans: Disease markers, Vol. 5, Issue 4, pp. 215-25, (1988) (PubMed).
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Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells." dans: Blood, Vol. 105, Issue 3, pp. 1256-64, (2005) (PubMed).
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- Antigène
- CD33
- Autre désignation
- CD33 (CD33 Produits)
- Synonymes
- anticorps SIGLEC-3, anticorps SIGLEC3, anticorps p67, anticorps Siglec-3, anticorps gp67, anticorps CD33 molecule, anticorps CD33 antigen, anticorps CD33, anticorps Cd33
- Sujet
- CD33 Molecule,CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.,SIGLEC3, p67
- ID gène
- 945
- UniProt
- P20138
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