Streptavidin anticorps

N° du produit ABIN398439

Détail du produit anti-Streptavidin anticorps

Antigène: Streptavidin

Reactivité: Streptomyces avidinii

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Streptavidin est non-conjugé

Application: ELISA, Western Blotting (WB)

Specificité: Streptavidin Antibody is specific to streptavidin.

Purification: Protein G chromatography

Immunogène: Purified recombinant streptavidin

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.5-1 µg/mL Dot blot: 0.5-1 µg/mL Western blot: 0.5-1 µg/mL Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH7.4, containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Références pour anticorps anti-Streptavidin (ABIN398439)

Snyder, Kellogg, Skarda, Little, Kristich: "Nutritional control of antibiotic resistance via an interface between the phosphotransferase system and a two-component signaling system." dans: Antimicrobial agents and chemotherapy, Vol. 58, Issue 2, pp. 957-65, (2014) (PubMed).

Lorey, Fiedler, Kunert, Nerkamp, Lange, Fiedler, Bosse-Doenecke, Meysing, Gloser, Rundfeldt, Rauchhaus, Hänssgen, Göttler, Steuernagel, Fiedler, Haupts: "Novel ubiquitin-derived high affinity binding proteins with tumor targeting properties." dans: The Journal of biological chemistry, Vol. 289, Issue 12, pp. 8493-507, (2014) (PubMed).

Yonekita, Ohtsuki, Hojo, Morishita, Matsumoto, Aizawa, Morimatsu: "Development of a novel multiplex lateral flow assay using an antimicrobial peptide for the detection of Shiga toxin-producing Escherichia coli." dans: Journal of microbiological methods, Vol. 93, Issue 3, pp. 251-6, (2013) (PubMed).

Détails sur Streptavidin

Antigène: Streptavidin

Abstract: Streptavidin Produits

Sujet: Streptavidin is a tetrameric protein from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of almost equal to10-14 mol/L. The high-affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Streptavidin Antibody is developed in rabbit using purified Streptavidin and highly purified from rabbit antiserum by protein G resin.