CD45 anticorps (FITC)

N° du produit ABIN793466

Détail du produit anti-CD45 anticorps (FITC)

Antigène: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD45 est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Specificité: Human PTPRC / CD45

Purification: Protein A/G purified

Stérilité: Sterile filtered

Immunogène: Peripheral blood monocytes from rheumatoid arthritis patient.

Clone: BHPT-1

Isotype: IgG1

Information d'application

Indications d'application: Approved: Flo

Usage: PBMC: Add 10 μL of antibody/10^6 PBMC in 100 μL PBS. Mix gently and incubate for 15 minutes at 2 to 8 °C. Wash twice with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 μL of antibody/100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20 °C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5+: As above, however use only 5 μL per test rather than 10 l. Excites at 488nm and emits at 670nm. Store protected from light. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.08 % sodium azide, 0.2 % carrier protein, sterile-filtered

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 4°C. Do not freeze.

Détails sur CD45

Antigène: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Autre désignation: CD45 (PTPRC Produits)

Synonymes: anticorps PTPRC, anticorps si:dkeyp-47c5.1, anticorps B220, anticorps CD45, anticorps CD45R, anticorps GP180, anticorps L-CA, anticorps LCA, anticorps LY5, anticorps T200, anticorps Cd45, anticorps Ly-5, anticorps Lyt-4, anticorps loc, anticorps Lca, anticorps RT7, anticorps cd45, anticorps protein tyrosine phosphatase, receptor type C, anticorps protein tyrosine phosphatase, receptor type, C, anticorps receptor-type tyrosine-protein phosphatase C, anticorps PTPRC, anticorps ptprc, anticorps LOC100074271, anticorps Ptprc

Sujet: Name/Gene ID: PTPRC
Subfamily: R1/R6
Family: Protein Phosphatase

Synonyms: PTPRC, B220, CD45R, CD45, gp180, Leukocyte common antigen, L-CA, Leucocyte common antigen, LY5, T200, T200 glycoprotein, T200 leukocyte common antigen, CD45 antigen, LCA

ID gène: 5788

UniProt: P08575

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling