AQP2 anticorps (C-Term)

N° du produit ABIN863207

Détail du produit anti-AQP2 anticorps

Antigène: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Épitope: C-Term

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AQP2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: Detects ~28.8 kDa. May detect larger glycosylated bands ~35-50 kDa.

Réactivité croisée: Humain, Souris, Rat

Purification: Protein A Purified

Immunogène: Produced against the C-terminal peptide (Sequence N-CLKGLEPDTDWEEREVRRRQ) of rat aquaporin 2

Information d'application

Indications d'application:

• WB (1:2000)
• IHC (1:200)
• ICC/IF (1:400)
• optimal dilutions for assays should be determined by the user.

Commentaires:

0.5 μg/ml of ABIN863207 was sufficient for detection of aquaporin 2 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Références pour anticorps anti-AQP2 (ABIN863207)

Sim, Himmel, Redd, Pulous, Rogers, Black, Hong, von Bergen, Blount: "Absence of PKC-alpha attenuates lithium-induced nephrogenic diabetes insipidus." dans: PLoS ONE, Vol. 9, Issue 7, pp. e101753, (2014) (PubMed).

van der Lubbe, Lim, Meima, van Veghel, Rosenbaek, Mutig, Danser, Fenton, Zietse, Hoorn: "Aldosterone does not require angiotensin II to activate NCC through a WNK4-SPAK-dependent pathway." dans: Pflügers Archiv : European journal of physiology, Vol. 463, Issue 6, pp. 853-63, (2012) (PubMed).

Détails sur AQP2

Antigène: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Autre désignation: Aquaporin 2 (AQP2 Produits)

Synonymes: anticorps AQP-CD, anticorps WCH-CD, anticorps cph, anticorps jpk, anticorps AQP-2, anticorps aquaporin-2, anticorps aquaporin 2, anticorps aquaporin 2 (collecting duct), anticorps Aqp2, anticorps AQP2

Sujet: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. It is located in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed vasopressin binds to the cell surface vasopressin receptor, activating a signaling pathway that cause AQP2 containing vesicles to fuse with the plasma membrane so the AQP2 can be used by the cell (3). Defects in AQP2 area cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI) (4).

ID gène: 25386

NCBI Accession: NP_037041

UniProt: P34080

Pathways: Response to Water Deprivation