Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificité
The antibody detects endogenous level of total STAT3 protein.
Purification
Affinity purified
Immunogène
Peptide sequence around AA 703-707 (A-P-Y-L-K) derived from Human STAT3. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Immunofluorescence: IF:1:100-1:200
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Cheng, Wang, Xia, Yan, Tang, Chen, Zhang, Liu, Kong, Sjöberg, Folco, Libby, Liao, Shi: "A guanidine-rich regulatory oligodeoxynucleotide improves type-2 diabetes in obese mice by blocking T-cell differentiation." dans: EMBO molecular medicine, Vol. 4, Issue 10, pp. 1112-25, (2012) (PubMed).
Takeichi, Sugiura, Muro, Matsumoto, Ogawa, Futamura, Kaminuma, Hashimoto, Shimoyama, Saito, Tomita: "Overexpression of LEDGF/DFS70 induces IL-6 via p38 activation in HaCaT cells, similar to that seen in the psoriatic condition." dans: The Journal of investigative dermatology, Vol. 130, Issue 12, pp. 2760-7, (2010) (PubMed).
García-Prieto, González-López, Cabrera, Astudillo, Gutiérrez-Fernández, Fanjul-Fernandez, Batalla-Solís, Puente, Fueyo, López-Otín, Albaiceta: "Resistance to bleomycin-induced lung fibrosis in MMP-8 deficient mice is mediated by interleukin-10." dans: PLoS ONE, Vol. 5, Issue 10, pp. e13242, (2010) (PubMed).
Antigène
STAT3
(Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))