CHEK2 anticorps (pSer516) (AbBy Fluor® 488)
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- Antigène Voir toutes CHEK2 Anticorps
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
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Épitope
- pSer516
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp CHEK2 est conjugé à/à la AbBy Fluor® 488
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Application
- Western Blotting (WB)
- Réactivité croisée
- Humain
- Homologie
- Mouse,Rat,Dog,Pig,Chicken,Rabbit
- Purification
- Purified by Protein A.
- Immunogène
- KLH conjugated synthetic phosphopeptide derived from human CHK2 around the phosphorylation site of ser516
- Isotype
- IgG
- Top Product
- Discover our top product CHEK2 Anticorps primaire
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- Indications d'application
- IF(IHC-P) 1:50-200
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
- Autre désignation
- Chk2 (CHEK2 Produits)
- Synonymes
- anticorps CDS1, anticorps CHK2, anticorps HuCds1, anticorps LFS2, anticorps PP1425, anticorps RAD53, anticorps hCds1, anticorps fa66f08, anticorps wu:fa66f08, anticorps zgc:55865, anticorps Cds1, anticorps HUCDS1, anticorps Rad53, anticorps Chk2, anticorps cds1, anticorps chek2, anticorps chk2, anticorps hucds1, anticorps lfs2, anticorps pp1425, anticorps rad53, anticorps checkpoint kinase 2, anticorps serine/threonine-protein kinase chk2, anticorps checkpoint kinase 2 L homeolog, anticorps CHEK2, anticorps chek2, anticorps Chek2, anticorps MCYG_07308, anticorps chek2.L
- Sujet
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Synonyms: Chk2 Ser516, bA444G7, CHK2 checkpoint homolog, CHK2_HUMAN, Serine/threonine-protein kinase Chk2, CDS 1, CDS1, Checkpoint kinase 2, Checkpoint like protein CHK2, Chek 2, Chek2, Chk 2, CHK2 checkpoint homolog S. pombe, CHK2 checkpoint homolog, HuCds 1, HuCds1, LFS 2, LFS2, PP1425, RAD 53, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2.
Background: Chk2 is a serine/threonine kinase involved in the control of cell cycle checkpoints, and may also participate in transduction of the DNA damage and replicational stress signals. Chk2 is the mammalian ortholog of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases. The amino-terminal domain of Chk2 contains a series of seven serine and threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases. Indeed, after DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain. Chk2 inhibits CDC25C phosphatase by phosphorylating it on Ser-216, preventing the entry into mitosis. This kinase may have a role in meiosis as well. Kinase activity is up regulated by autophosphorylation and the protein is rapidly phosphorylated in response to DNA damage and to replication block.
- ID gène
- 11200
- Pathways
- Signalisation p53, Apoptose, Cycle Cellulaire
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