Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

RAG1 anticorps (AA 351-450) (AbBy Fluor® 488)

RAG1 Reactivité: Souris WB, FACS, IF (cc), IF (p) Hôte: Lapin Polyclonal AbBy Fluor® 488
N° du produit ABIN912873
  • Antigène Voir toutes RAG1 Anticorps
    RAG1 (Recombination Activating Gene 1 (RAG1))
    Épitope
    • 15
    • 3
    • 1
    • 1
    • 1
    AA 351-450
    Reactivité
    • 18
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Hôte
    • 20
    • 1
    Lapin
    Clonalité
    • 20
    • 1
    Polyclonal
    Conjugué
    • 7
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp RAG1 est conjugé à/à la AbBy Fluor® 488
    Application
    • 21
    • 13
    • 12
    • 12
    • 4
    • 3
    • 3
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
     Réactivité croisée
    Souris
    Homologie
    Human,Rat,Sheep,Pig
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from human RAG1/RNF74
    Isotype
    IgG
    Top Product
    Discover our top product RAG1 Anticorps primaire
  • Indications d'application
    FCM 1:20-100
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    RAG1 (Recombination Activating Gene 1 (RAG1))
    Autre désignation
    RNF74 (RAG1 Produits)
    Synonymes
    anticorps RAG-1, anticorps rag1, anticorps RNF74, anticorps Rag-1, anticorps rag-1, anticorps rag1a, anticorps recombination activating 1, anticorps recombination activating gene 1, anticorps recombination activating gene 1 S homeolog, anticorps Rag1, anticorps RAG1, anticorps rag1, anticorps rag1.S
    Sujet

    Synonyms: RAG-1, RNF74, V(D)J recombination-activating protein 1, RING finger protein 74, RAG1

    Background: Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination.

    ID gène
    5896
    UniProt
    P15918
Vous êtes ici:
Support technique