The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Vennegoor, Hageman, Van Nouhuijs, Ruiter, Calafat, Ringens, Rümke: "A monoclonal antibody specific for cells of the melanocyte lineage." dans: The American journal of pathology, Vol. 130, Issue 1, pp. 179-92, (1988) (PubMed).