EXOS anticorps (cleaved)

N° du produit ABIN93557

Détail du produit anti-EXOS anticorps

Antigène: EXOS (ExoS Gene Product (EXOS))

Épitope: cleaved

Reactivité: Pseudomonas aeruginosa

Hôte: Poulet

Clonalité: Polyclonal

Application: Western Blotting (WB)

Réactivité croisée (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Homologie: Pseudomonas aeruginosa only

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 48 kDa

Purification: affinity purified

Immunogène: amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.

Isotype: IgY

Information d'application

Indications d'application: 1: 5000 (WB)

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS pH 8.0+ 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Make aliquots to avoid repreated freeze-thaw cycles and working with a stock.

Stock: 4 °C

Stockage commentaire: store at 4°C, make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Références pour anticorps anti-EXOS (ABIN93557)

Shen, Tsay, Chia, Wu, Lee, Hu: "The Role of Type III Secretion System and Lens Material on the Adhesion of Pseudomonas aeruginosa to Contact Lenses." dans: Investigative ophthalmology & visual science, (2012) (PubMed).

Détails sur EXOS

Antigène: EXOS (ExoS Gene Product (EXOS))

Autre désignation: Exoenzyme S (ExoS)

Sujet: Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.

Poids moléculaire: 48 kDa

UniProt: Q51449