TUBB anticorps

N° du produit ABIN93914

Détail du produit anti-TUBB anticorps

Antigène: TUBB (Tubulin, beta (TUBB))

Reactivité: Humain, Souris, Rat, Porc, Poulet, Tetrahymena, Poisson, Paramecium, Nicotiana tabacum, Arabidopsis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TUBB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificité: The antibody TU-06 recognizes an epitope (aa 81-95) on phylogenetically conserved N-terminal structural domain of beta-tubulin (recognizes all beta-tubulin isoforms) in various species.

Réactivité croisée (Details): Broad species reactivity

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Beta-subunits of porcine brain tubulin.

Clone: TU-06

Isotype: IgM

Information d'application

Indications d'application: Immunocytochemistry: Recommended dilution: 2 μg/mL, fixed and permeabilized cells, positive control: 3T3 mouse embryonal fibroblast cell line.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive tissue: heart.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-TUBB (ABIN93914)

Tobita, Liu, Janczewski, Tinney, Nonemaker, Augustine, Stolz, Shroff, Keller: "Engineered early embryonic cardiac tissue retains proliferative and contractile properties of developing embryonic myocardium." dans: American journal of physiology. Heart and circulatory physiology, Vol. 291, Issue 4, pp. H1829-37, (2006) (PubMed).

Libusová, Sulimenko, Sulimenko, Janisch, Hozák, Dráber: "Distinct localization of a beta-tubulin epitope in the Tetrahymena thermophila and Paramecium caudatum cortex." dans: Protoplasma, Vol. 225, Issue 3-4, pp. 157-67, (2005) (PubMed).

Solecki, Model, Gaetz, Kapoor, Hatten: "Par6alpha signaling controls glial-guided neuronal migration." dans: Nature neuroscience, Vol. 7, Issue 11, pp. 1195-203, (2004) (PubMed).

Smertenko, Blume, Viklický, Opatrný, Dráber: "Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells." dans: Planta, Vol. 201, Issue 3, pp. 349-58, (1997) (PubMed).

Smertenko, Blume, Viklický, Dráber: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." dans: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, (1997) (PubMed).

Dráber, Dráberová, Viklický: "Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head." dans: Histochemistry, Vol. 95, Issue 5, pp. 519-24, (1991) (PubMed).

Détails sur TUBB

Antigène: TUBB (Tubulin, beta (TUBB))

Autre désignation: beta-tubulin (TUBB Produits)

Synonymes: anticorps M40, anticorps OK/SW-cl.56, anticorps TUBB1, anticorps TUBB5, anticorps XLOT, anticorps m40, anticorps ok/sw-cl.56, anticorps tubb1, anticorps tubb5, anticorps TUBB2A, anticorps TUBB2B, anticorps TUBB, anticorps 1t, anticorps B1t, anticorps BETA 56D, anticorps CG9277, anticorps DTB2, anticorps Dmbeta1, anticorps Dmel\\CG9277, anticorps T, anticorps Tub, anticorps Tubulin, anticorps beta, anticorps beta-Tub, anticorps beta-Tub56D, anticorps beta-tub, anticorps beta-tubulin56D, anticorps beta1, anticorps beta1-Tubulin, anticorps beta1-tub, anticorps beta1Tub, anticorps beta1t, anticorps beta1tub, anticorps beta56D, anticorps betaTub, anticorps betaTub1, anticorps beta[[1]] tubulin, anticorps beta[[1]]-tubulin, anticorps betatub(56D), anticorps 143391_i_at, anticorps 3t, anticorps B3t, anticorps BETA 60D, anticorps CG3401, anticorps D.m.BETA-60D, anticorps DTB3, anticorps Dmbeta3, anticorps Dmel\\CG3401, anticorps Tub60D, anticorps beta-Tub60D, anticorps beta-Tub6D, anticorps beta3, anticorps beta3 TU, anticorps beta3-Tub, anticorps beta3-tubulin, anticorps beta3TUB, anticorps beta3Tub, anticorps beta3t, anticorps beta60C, anticorps betaTub3, anticorps betaTub60C, anticorps beta[[3]] tubulin, anticorps beta[[3]]-Tub, anticorps beta[[3]]-tubulin, anticorps betatub60D, anticorps p50, anticorps p50/tubulin, anticorps p53, anticorps p53/tubulin, anticorps 2t, anticorps B2t, anticorps BETA 85D, anticorps BETA2, anticorps CG9359, anticorps D.m.BETA-85D, anticorps DTB4, anticorps Dmbeta2, anticorps Dmel\\CG9359, anticorps beta(2)Tu, anticorps beta(2)Tub, anticorps beta-Tub85D, anticorps beta-tub85D, anticorps beta2, anticorps beta2-tub, anticorps beta2t, anticorps beta2tub, anticorps beta85D, anticorps betaTub2, anticorps beta[[2]]-tubulin, anticorps ms(3)KK[D], anticorps 4t, anticorps B4t, anticorps BETA 98B, anticorps CG4869, anticorps DTB1, anticorps Dmbeta4, anticorps Dmel\\CG4869, anticorps beta-Tub97EF, anticorps beta4, anticorps beta4t, anticorps beta97F, anticorps betaTub4, anticorps betaTub98BC, anticorps betaTub98C, anticorps Tub1, anticorps bmtub1, anticorps Tub3, anticorps bmtub3, anticorps Tub2, anticorps bmtub2, anticorps Tub4, anticorps bmtub4, anticorps LOC100101153, anticorps tubulin beta class I, anticorps putative cobalt-zinc-cadmium outer membrane resistance protein, anticorps Tubulin beta chain, anticorps tubulin beta-2A chain, anticorps beta-Tubulin at 56D, anticorps beta-Tubulin at 60D, anticorps beta-Tubulin at 85D, anticorps beta-Tubulin at 97EF, anticorps beta-tubulin, anticorps tubulin beta 6 class V, anticorps beta tubulin, anticorps tubulin beta class I L homeolog, anticorps tubulin, beta 5 class I, anticorps TUBB, anticorps czcC, anticorps tbb-6, anticorps tubb, anticorps LOC462399, anticorps betaTub56D, anticorps betaTub60D, anticorps betaTub85D, anticorps betaTub97EF, anticorps Tub1, anticorps Tub3, anticorps Tub2, anticorps Tub4, anticorps TVAG_525430, anticorps TVAG_062880, anticorps TVAG_456920, anticorps LOC100101153, anticorps TUBB6, anticorps Tb927.1.2330, anticorps Tb927.1.2350, anticorps Tb927.1.2370, anticorps Tb927.1.2390, anticorps tubb.L, anticorps Tubb, anticorps LOC693049, anticorps LOC443015, anticorps Tubb5

Sujet: Tubulin beta,The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer. It is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.,TUBB

ID gène: 81027

UniProt: Q9H4B7

Pathways: Dynamique des Microtubules, M Phase