CD11b anticorps

N° du produit ABIN93956

Détail du produit anti-CD11b anticorps

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD11b est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificité: The antibody MEM-174 recognizes an extracellular epitope of CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Human granulocytes

Clone: MEM-174

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1 μg/mL, positive control: peripheral blood lymphocytes.
Immunoprecipitation: Positive control: granulocytes, CD11b/CD18 transfectants.
Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 mL of sorbent gel (e.g. agarose beads). It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD11b (ABIN93956)

Hasan, Osickova, Bumba, Novák, Sebo, Osicka: "Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding." dans: FEBS letters, Vol. 589, Issue 3, pp. 374-9, (2015) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." dans: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Détails sur CD11b

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Autre désignation: CD11b (ITGAM Produits)

Synonymes: anticorps CD11b/CD18, anticorps CR3, anticorps CR3A, anticorps Cd11b, anticorps F730045J24Rik, anticorps Ly-40, anticorps MAC1, anticorps Mac-1, anticorps Mac-1a, anticorps CD11B, anticorps MAC-1, anticorps MAC1A, anticorps MO1A, anticorps SLEB6, anticorps C3bi, anticorps integrin subunit alpha M, anticorps integrin alpha M, anticorps integrin, alpha M (complement component 3 receptor 3 subunit), anticorps integrin alpha-M, anticorps ITGAM, anticorps Itgam, anticorps LOC100351865

Sujet: Integrin subunit alpha M,CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.,Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A

ID gène: 3684

UniProt: P11215

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation