CD14 anticorps
-
- Antigène Voir toutes CD14 Anticorps
- CD14
-
Reactivité
- Humain, Non-Human Primate
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp CD14 est non-conjugé
-
Application
- Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Specificité
- The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked extracellular membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes, also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.
- Réactivité croisée (Details)
- Non-Human Primates, Human
- Purification
- Purified by protein-A affinity chromatography.
- Pureté
- > 95 % (by SDS-PAGE)
- niveau d'endotoxine
- Low Endotoxin
- Immunogène
- A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
- Clone
- MEM-18
- Isotype
- IgG1
- Top Product
- Discover our top product CD14 Anticorps primaire
-
-
- Indications d'application
-
ELISA: The antibody MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody B-A8 (cat. no. 11-304-C100).
Flow cytometry: recommended dilution: 4 μg/mL.
Western blotting: Non-reducing conditions. - Restrictions
- For Research Use only
-
- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Do not freeze.
- Stock
- 4 °C
- Stockage commentaire
- Store at 2-8°C. Do not freeze.
-
-
Hepatic progenitor cells from adult human livers for cell transplantation." dans: Gut, Vol. 57, Issue 8, pp. 1129-38, (2008) (PubMed).
: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." dans: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).
: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." dans: International immunology, Vol. 19, Issue 11, pp. 1271-9, (2007) (PubMed).
: "The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain." dans: Biochemical and biophysical research communications, Vol. 328, Issue 1, pp. 173-6, (2005) (PubMed).
: "Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression." dans: The Journal of experimental medicine, Vol. 196, Issue 8, pp. 1017-24, (2002) (PubMed).
: "CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited." dans: Infection and immunity, Vol. 69, Issue 6, pp. 3772-81, (2001) (PubMed).
: "Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14." dans: Molecular immunology, Vol. 26, Issue 7, pp. 657-62, (1989) (PubMed).
: "Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen." dans: European journal of immunology, Vol. 16, Issue 12, pp. 1583-9, (1987) (PubMed).
: "
-
Hepatic progenitor cells from adult human livers for cell transplantation." dans: Gut, Vol. 57, Issue 8, pp. 1129-38, (2008) (PubMed).
-
- Antigène
- CD14
- Autre désignation
- CD14 (CD14 Produits)
- Synonymes
- anticorps CD14 molecule, anticorps CD14 antigen, anticorps CD14, anticorps Cd14
- Sujet
- CD14 Molecule,CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.,LPS-receptor, LPSR, MCLR
- ID gène
- 929
- UniProt
- P08571
- Pathways
- Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
-