CD43 anticorps

N° du produit ABIN94116

Détail du produit anti-CD43 anticorps

Antigène: CD43 (SPN) (Sialophorin (SPN))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD43 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificité: The antibody MEM-59 recognizes a neuraminidase-sensitive extracellular epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes, it is negative on resting B lymphocytes and erythrocytes.

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Human T lymphocytes.

Clone: MEM-59

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1-5 μg/mL.
Immunohistochemistry (paraffin sections): Positive tissue: spleen, thymus, tonsil, recommended dilution: 10 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD43 (ABIN94116)

Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." dans: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

Símová, Klíma, Cermak, Sourková, Andera: "Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane." dans: Apoptosis : an international journal on programmed cell death, Vol. 13, Issue 3, pp. 423-36, (2008) (PubMed).

Filatov, Krotov, Zgoda, Volkov: "Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry." dans: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 21-33, (2007) (PubMed).

Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." dans: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, (2002) (PubMed).

Alvarado, Klassen, Cerny, Horejsí, Schmidt: "MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation." dans: European journal of immunology, Vol. 25, Issue 4, pp. 1051-5, (1995) (PubMed).

Stefanová, Hilgert, Angelisová, Kristofová, Horejsí: "Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin." dans: Folia biologica, Vol. 34, Issue 4, pp. 255-65, (1989) (PubMed).

Détails sur CD43

Antigène: CD43 (SPN) (Sialophorin (SPN))

Autre désignation: CD43 (SPN Produits)

Synonymes: anticorps CD43, anticorps GALGP, anticorps GPL115, anticorps LSN, anticorps A630014B01Rik, anticorps Cd43, anticorps Galgp, anticorps Ly-48, anticorps Ly48, anticorps Lsn, anticorps Lsn1, anticorps gpL115, anticorps sialophorin, anticorps SPN, anticorps Spn

Sujet: Sialophorin,CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.,Leukosialin, Sialophorin, Galactoglycoprotein, GALGP, LSN, SPN, GALGP, GP5

ID gène: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity