CD63 anticorps (FITC)

N° du produit ABIN94215

Détail du produit anti-CD63 anticorps (FITC)

Antigène: CD63

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD63 est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Specificité: The antibody MEM-259 reacts with an extracellular/luminal epitope of CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.

Réactivité croisée (Details): Human

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: HPB-ALL T cell line

Clone: MEM-259

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD63 (FITC) (ABIN94215)

Heneberg, Riegerová, Kučera: "Pimecrolimus Is a Potent Inhibitor of Allergic Reactions to Hymenopteran Venom Extracts and Birch Pollen Allergen In Vitro." dans: PLoS ONE, Vol. 10, Issue 11, pp. e0142953, (2015) (PubMed).

Cerny, Feng, Yu, Miyake, Borgonovo, Klumperman, Meldolesi, McNeil, Kirchhausen: "The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing." dans: EMBO reports, Vol. 5, Issue 9, pp. 883-8, (2004) (PubMed).

Détails sur CD63

Antigène: CD63

Autre désignation: CD63 (CD63 Produits)

Synonymes: anticorps C75951, anticorps ME491, anticorps Tspan30, anticorps LAMP-3, anticorps MLA1, anticorps OMA81H, anticorps TSPAN30, anticorps TSP29FA, anticorps CD63 antigen, anticorps CD63 molecule, anticorps Cd63 molecule, anticorps Cd63, anticorps CD63

Sujet: CD63 Molecule,CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.,OMA81H, Granulophysin, Tetraspanin-30, Tspan-30, MLA1, ME491, LAMP-3, OMA81H, TSPAN30

ID gène: 967

UniProt: P08962