CD8 anticorps

N° du produit ABIN94232

Détail du produit anti-CD8 anticorps

Antigène: CD8

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Cytometry by Time of Flight (CyTOF)

Specificité: The antibody MEM-31 recognizes a conformationally-dependent extracellular epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa). The antibody does not react with formaldehyde-fixed cells, negative in Western blotting application.

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Crude thymus membrane fraction.

Clone: MEM-31

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD8 (ABIN94232)

Linnebacher, Wienck, Boeck, Klar: "Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO)." dans: Journal of biomedicine & biotechnology, Vol. 2010, pp. 841451, (2010) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." dans: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Estefanía, Flores, Gómez-Lozano, Aguilar, López-Botet, Vilches: "Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 7, pp. 4402-10, (2007) (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." dans: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).

Détails sur CD8

Antigène: CD8

Autre désignation: CD8 (CD8 Produits)

Synonymes: anticorps CD8, anticorps Leu2, anticorps MAL, anticorps p32, anticorps CD8a molecule, anticorps CD8A

Sujet: The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.,p32, LEU2