CD80 anticorps (Extracellular Domain) (APC)

N° du produit ABIN94241

Détail du produit anti-CD80 anticorps (APC)

Antigène: CD80

Épitope: Extracellular Domain

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD80 est conjugé à/à la APC

Application: Flow Cytometry (FACS)

Specificité: The antibody MEM-233 reacts with an extracellular epitope of CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

Réactivité croisée (Details): Human

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: Extracellular domain of human CD80 fused to human IgG1(Fc)

Clone: MEM-233

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD80 (APC) (ABIN94241)

Silk, Leishman, Nishimoto, Reddy, Fairchild: "Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype." dans: Journal of biomedicine & biotechnology, Vol. 2012, pp. 172420, (2012) (PubMed).

Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." dans: BMC immunology, Vol. 12, pp. 2, (2011) (PubMed).

Lee, Sieling, Ochoa, Krutzik, Guo, Hernandez, Rea, Cheng, Colonna, Modlin: "LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 12, pp. 8128-36, (2007) (PubMed).

Kolar, Mehta, Pelayo, Capra: "A novel human B cell subpopulation representing the initial germinal center population to express AID." dans: Blood, Vol. 109, Issue 6, pp. 2545-52, (2007) (PubMed).

Campioni, Moretti, Ferrari, Punturieri, Castoldi, Lanza: "Immunophenotypic heterogeneity of bone marrow-derived mesenchymal stromal cells from patients with hematologic disorders: correlation with bone marrow microenvironment." dans: Haematologica, Vol. 91, Issue 3, pp. 364-8, (2006) (PubMed).

Tan, Beutelspacher, Wang, McClure, Ritter, Lombardi, George: "Immunolipoplexes: an efficient, nonviral alternative for transfection of human dendritic cells with potential for clinical vaccination." dans: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 11, Issue 5, pp. 790-800, (2005) (PubMed).

Zhan, Towler, Calder: "The immunomodulatory role of human conjunctival epithelial cells." dans: Investigative ophthalmology & visual science, Vol. 44, Issue 9, pp. 3906-10, (2003) (PubMed).

Détails sur CD80

Antigène: CD80

Autre désignation: CD80 (CD80 Produits)

Synonymes: anticorps B7, anticorps B7-1, anticorps B7.1, anticorps BB1, anticorps CD28LG, anticorps CD28LG1, anticorps LAB7, anticorps B71, anticorps Cd28l, anticorps Ly-53, anticorps Ly53, anticorps MIC17, anticorps TSA1, anticorps CD80 molecule, anticorps Cd80 molecule, anticorps CD80 antigen, anticorps CD80, anticorps Cd80

Sujet: CD80 Molecule,CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 Molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet, there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.,B7-1, BB1

ID gène: 941

UniProt: P33681

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints