Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)
Specificité
The antibody IVA50 reacts with an extracellular epitope of CD9 antigen (bovine), a 24 kDa single transmembrane polypeptide expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes. This antibody crossreacts with CD9 on human platelets.
CD9
Reactivité: Humain
FACS, WB, ELISA, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western blotting: Recommended dilution: 1 μg/mL.
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do not freeze.
Stock
4 °C
Stockage commentaire
Store at 2-8°C. Do not freeze.
de Pontes, Altei, Galan, Bilić, Guillemin, Kuleš, Horvatić, Ribeiro, de Paula, Pereira, Lucheis, Mrljak, Eckersall, Ferreira, Dos Santos: "Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes." dans: The journal of venomous animals and toxins including tropical diseases, Vol. 26, pp. e20190067, (2020) (PubMed).
Tomásková, Dusinský, Horovská, Simon: "A set of monoclonal antibodies specific for bovine cell surface molecule CD9." dans: Folia biologica, Vol. 45, Issue 5, pp. 225-6, (2000) (PubMed).
Mateo, Perez de la Lastra, Moreno, Dusinsky, Bilka, Simon, Horovska, Llanes: "Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies." dans: Veterinary immunology and immunopathology, Vol. 52, Issue 4, pp. 363-70, (1997) (PubMed).
Naessens, Howard, Hopkins: "Nomenclature and characterization of leukocyte differentiation antigens in ruminants." dans: Immunology today, Vol. 18, Issue 8, pp. 365-8, (1997) (PubMed).
Sopp, Howard: "Investigation of PC36 (BoCD45R)." dans: Veterinary immunology and immunopathology, Vol. 52, Issue 4, pp. 271-4, (1997) (PubMed).
CD9 Molecule,CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.,MIC3, MRP-1, BTCC-1, DRAP-27, TSPAN29