Keratin 7/17 anticorps

N° du produit ABIN94296

Détail du produit anti-Keratin 7/17 anticorps

Antigène: Keratin 7/17 (KRT7/17)

Reactivité: Humain, Boeuf (Vache), Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Keratin 7/17 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: The antibody C-46 reacts with cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues.

Aucune reactivité croisée: Souris, Lapin, Rat

Réactivité croisée (Details): Porcine, Human, Bovine

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Cytoskeleton preparation of HeLa human cervix carcinoma cell line.

Clone: C-46

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, pretreatment: 0,1 % pepsin, 30 min, 37 °C, incubation 4 °C overnight, chromogen DAB, positive tissue: pancreas.
Western blotting: Recommended dilution: 1-2 μg/mL, positive control: HeLa cells.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-Keratin 7/17 (ABIN94296)

Vojt?sek, Stasková, Nenutil, Bártková, Kovarík, Rejthar, Bártek: "A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis." dans: Neoplasma, Vol. 37, Issue 3, pp. 333-42, (1990) (PubMed).

Détails sur Keratin 7/17

Antigène: Keratin 7/17 (KRT7/17)

Autre désignation: Cytokeratin 7+17 (KRT7/17 Produits)

Synonymes: anticorps KRT7, anticorps CK7, anticorps keratin 7, anticorps KRT7

Sujet: Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K7, K17, CK7, CD17, CK17, CYK7, CYK17, KRT7, KRT17, SCL, K2C7