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MICA anticorps

MICA Reactivité: Humain WB, FACS, IP Hôte: Souris Monoclonal MEM-147 unconjugated
N° du produit ABIN94329
  • Antigène Voir toutes MICA Anticorps
    MICA (MHC Class I Polypeptide-Related Sequence A (MICA))
    Reactivité
    • 69
    • 18
    • 14
    • 11
    • 7
    • 5
    • 2
    • 1
    Humain
    Hôte
    • 37
    • 32
    Souris
    Clonalité
    • 37
    • 33
    Monoclonal
    Conjugué
    • 44
    • 8
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp MICA est non-conjugé
    Application
    • 44
    • 36
    • 33
    • 18
    • 17
    • 12
    • 9
    • 8
    • 6
    • 5
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)
    Specificité
    The antibody MEM-147 reacts with an extracellular epitope of all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
    Réactivité croisée (Details)
    Human
    Purification
    Purified by protein-A affinity chromatography.
    Pureté
    > 95 % (by SDS-PAGE)
    Immunogène
    PHA-activated peripheral blood lymphocytes
    Clone
    MEM-147
    Isotype
    IgG1
    Top Product
    Discover our top product MICA Anticorps primaire
  • Indications d'application
    Flow cytometry: Recommended dilution: 1-5 μg/mL.
    Western blotting: Non-reducing conditions.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Do not freeze.
  • Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." dans: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

    Tran, Ivanyi, Hilgert, Brdicka, Pla, Breur, Flieger, Ivasková, Horejsí: "The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex." dans: Immunogenetics, Vol. 53, Issue 6, pp. 440-6, (2001) (PubMed).

    Ilangumaran, Briol, Hoessli: "CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes." dans: Blood, Vol. 91, Issue 10, pp. 3901-8, (1998) (PubMed).

  • Antigène
    MICA (MHC Class I Polypeptide-Related Sequence A (MICA))
    Autre désignation
    HLA-Class I (MICA Produits)
    Synonymes
    anticorps MIC-A, anticorps PERB11.1, anticorps MHC class I polypeptide-related sequence A, anticorps MICA
    Sujet
    HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
    Pathways
    Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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