MICA anticorps

N° du produit ABIN94329

Détail du produit anti-MICA anticorps

Antigène: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MICA est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificité: The antibody MEM-147 reacts with an extracellular epitope of all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: PHA-activated peripheral blood lymphocytes

Clone: MEM-147

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1-5 μg/mL.
Western blotting: Non-reducing conditions.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-MICA (ABIN94329)

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." dans: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Tran, Ivanyi, Hilgert, Brdicka, Pla, Breur, Flieger, Ivasková, Horejsí: "The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex." dans: Immunogenetics, Vol. 53, Issue 6, pp. 440-6, (2001) (PubMed).

Ilangumaran, Briol, Hoessli: "CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes." dans: Blood, Vol. 91, Issue 10, pp. 3901-8, (1998) (PubMed).

Détails sur MICA

Antigène: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Autre désignation: HLA-Class I (MICA Produits)

Synonymes: anticorps MIC-A, anticorps PERB11.1, anticorps MHC class I polypeptide-related sequence A, anticorps MICA

Sujet: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response