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MICA anticorps (FITC)

MICA Reactivité: Humain, Boeuf (Vache), Cat, Non-Human Primate FACS Hôte: Souris Monoclonal W6-32 FITC
N° du produit ABIN94334
  • Antigène Voir toutes MICA Anticorps
    MICA (MHC Class I Polypeptide-Related Sequence A (MICA))
    Reactivité
    • 69
    • 17
    • 13
    • 11
    • 6
    • 5
    • 2
    • 1
    Humain, Boeuf (Vache), Cat, Non-Human Primate
    Hôte
    • 37
    • 32
    Souris
    Clonalité
    • 37
    • 33
    Monoclonal
    Conjugué
    • 45
    • 8
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp MICA est conjugé à/à la FITC
    Application
    • 45
    • 36
    • 33
    • 19
    • 17
    • 12
    • 9
    • 8
    • 6
    • 5
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificité
    The antibody W6/32 recognises an extracellular epitope of MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.
    Aucune reactivité croisée
    Lapin
    Réactivité croisée (Details)
    Human, Non-Human Primates, Bovine, Feline (Cat)
    Purification
    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogène
    Membrane of human tonsil cells
    Clone
    W6-32
    Isotype
    IgG2a
    Top Product
    Discover our top product MICA Anticorps primaire
  • Indications d'application
    Flow cytometry: Recommended dilution: 1-3 μg/mL.
    Commentaires

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Avoid prolonged exposure to light.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Le Discorde, Moreau, Sabatier, Legeais, Carosella: "Expression of HLA-G in human cornea, an immune-privileged tissue." dans: Human immunology, Vol. 64, Issue 11, pp. 1039-44, (2003) (PubMed).

    Tran, Ivanyi, Hilgert, Brdicka, Pla, Breur, Flieger, Ivasková, Horejsí: "The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex." dans: Immunogenetics, Vol. 53, Issue 6, pp. 440-6, (2001) (PubMed).

    Ladasky, Shum, Canavez, Seuánez, Parham: "Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody." dans: Immunogenetics, Vol. 49, Issue 4, pp. 312-20, (1999) (PubMed).

    Shields, Ribaudo: "Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin." dans: Tissue antigens, Vol. 51, Issue 5, pp. 567-70, (1998) (PubMed).

    Jacobsen, Aasted, Broe, Petersen: "Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species." dans: Veterinary immunology and immunopathology, Vol. 39, Issue 4, pp. 461-6, (1994) (PubMed).

    Stern, Beresford, Friedman, Stevens, Risk, Johnson: "Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 4, pp. 1088-91, (1987) (PubMed).

    Neefjes, Hensen, de Kroon, Ploegh: "A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes." dans: Immunogenetics, Vol. 23, Issue 5, pp. 341-7, (1986) (PubMed).

    Brodsky, Parham: "Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies." dans: Immunogenetics, Vol. 15, Issue 2, pp. 151-66, (1982) (PubMed).

    Barnstable, Bodmer, Brown, Galfre, Milstein, Williams, Ziegler: "Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis." dans: Cell, Vol. 14, Issue 1, pp. 9-20, (1978) (PubMed).

  • Antigène
    MICA (MHC Class I Polypeptide-Related Sequence A (MICA))
    Autre désignation
    HLA-Class I (MICA Produits)
    Synonymes
    anticorps MIC-A, anticorps PERB11.1, anticorps MHC class I polypeptide-related sequence A, anticorps MICA
    Sujet
    HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
    Pathways
    Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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