Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)
Specificité
The antibody HL-37 reacts with extracellular polymorphic determinant on human HLA-DQ1 and HLA-DQ3 Molecules (recognized epitope was found on isolated beta chain of DQ1), but does not react with HLA-DQ2. DQ is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).
Flow cytometry: Recommended dilution: 1-4 μg/mL. Western blotting: non-reducing conditions.
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do not freeze.
Stock
4 °C
Stockage commentaire
Store at 2-8°C. Do not freeze.
Horejsí, N?mec, Angelisová, Kristofová, Gorga, Hilgert: "Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens." dans: Tissue antigens, Vol. 28, Issue 5, pp. 288-97, (1987) (PubMed).
Antigène
HLA-DQ1/DQ3
(MHC Class II HLA-DQ1/DQ3 (HLA-DQ1/DQ3))
The antibody HL-37 reacts with polymorphic determinant on human HLA-DQ1 and HLA-DQ3 Molecules (recognized epitope was found on isolated beta chain of DQ1), but does not react with HLA-DQ2. DQ is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).