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HLAG anticorps (PE)

HLAG Reactivité: Humain FACS Hôte: Souris Monoclonal MEM-G-9 PE
N° du produit ABIN94373
  • Antigène Voir toutes HLAG Anticorps
    HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
    Reactivité
    • 126
    • 7
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 68
    • 63
    • 3
    • 1
    • 1
    Souris
    Clonalité
    • 73
    • 63
    Monoclonal
    Conjugué
    • 50
    • 19
    • 18
    • 14
    • 8
    • 7
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp HLAG est conjugé à/à la PE
    Application
    • 82
    • 65
    • 58
    • 44
    • 29
    • 25
    • 13
    • 13
    • 12
    • 10
    • 10
    • 6
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificité
    The antibody MEM-G/9 reacts with an extracellular epitope on native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
    Aucune reactivité croisée
    Souris
    Réactivité croisée (Details)
    Human
    Purification
    Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogène
    Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
    Clone
    MEM-G-9
    Isotype
    IgG1
    Top Product
    Discover our top product HLAG Anticorps primaire
  • Indications d'application
    Flow cytometry: Recommended dilution: 1-4 μg/mL, positive control: JEG-3 human choriocarcinoma cell line.
    Commentaires

    The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Avoid prolonged exposure to light.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Horii, Bui, Touma, Cho, Parast: "An Improved Two-Step Protocol for Trophoblast Differentiation of Human Pluripotent Stem Cells." dans: Current protocols in stem cell biology, Vol. 50, Issue 1, pp. e96, (2020) (PubMed).

    Spurny, Kailayangiri, Altvater, Jamitzky, Hartmann, Wardelmann, Ranft, Dirksen, Amler, Hardes, Fluegge, Meltzer, Farwick, Greune, Rossig: "T cell infiltration into Ewing sarcomas is associated with local expression of immune-inhibitory HLA-G." dans: Oncotarget, Vol. 9, Issue 5, pp. 6536-6549, (2018) (PubMed).

    Otti, Saleh, Velicky, Fiala, Pollheimer, Knöfler: "Notch2 controls prolactin and insulin-like growth factor binding protein-1 expression in decidualizing human stromal cells of early pregnancy." dans: PLoS ONE, Vol. 9, Issue 11, pp. e112723, (2014) (PubMed).

    Zhao, Teklemariam, Hantash: "Reassessment of HLA-G isoform specificity of MEM-G/9 and 4H84 monoclonal antibodies." dans: Tissue antigens, Vol. 80, Issue 3, pp. 231-8, (2012) (PubMed).

    López, Alegre, LeMaoult, Carosella, González: "Regulatory role of tryptophan degradation pathway in HLA-G expression by human monocyte-derived dendritic cells." dans: Molecular immunology, Vol. 43, Issue 14, pp. 2151-60, (2006) (PubMed).

    Gonen-Gross, Achdout, Arnon, Gazit, Stern, Horejsí, Goldman-Wohl, Yagel, Mandelboim: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, (2005) (PubMed).

    Menier, Saez, Horejsi, Martinozzi, Krawice-Radanne, Bruel, Le Danff, Reboul, Hilgert, Rabreau, Larrad, Pla, Carosella, Rouas-Freiss: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." dans: Human immunology, Vol. 64, Issue 3, pp. 315-26, (2003) (PubMed).

    Lozano, González, Kindelán, Rouas-Freiss, Caballos, Dausset, Carosella, Peña: "Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule." dans: AIDS, Vol. 16, Issue 3, pp. 347-51, (2002) (PubMed).

    Pangault, Le Friec, Caulet-Maugendre, Léna, Amiot, Guilloux, Onno, Fauchet: "Lung macrophages and dendritic cells express HLA-G molecules in pulmonary diseases." dans: Human immunology, Vol. 63, Issue 2, pp. 83-90, (2002) (PubMed).

    Fuzzi, Rizzo, Criscuoli, Noci, Melchiorri, Scarselli, Bencini, Menicucci, Baricordi: "HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy." dans: European journal of immunology, Vol. 32, Issue 2, pp. 311-5, (2002) (PubMed).

    Fournel, Huc, Aguerre-Girr, Solier, Legros, Praud-Brethenou, Moussa, Chaouat, Berrebi, Bensussan, Lenfant, Le Bouteiller: "Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules." dans: Tissue antigens, Vol. 55, Issue 6, pp. 510-8, (2000) (PubMed).

  • Antigène
    HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
    Autre désignation
    HLA-G (HLAG Produits)
    Synonymes
    anticorps MHC-G, anticorps B-F, anticorps B-F-S04, anticorps B-F-S05, anticorps B-F-S06, anticorps B-F-S07, anticorps B-FI, anticorps B-FIV, anticorps BF2, anticorps BFa2, anticorps BFw-03, anticorps BFw-05, anticorps BFz-01, anticorps major histocompatibility complex, class I, G, anticorps MHC BF1 class I, anticorps HLA-G, anticorps BF1
    Sujet
    Major histocompatibility complex, class I, G,Human leukocyte antigen G (HLA-G), belonging to MHC class I glycoproteins, plays important roles in both physiological and pathological immunotolerance. It gives an inhibitory signal to cytotoxic T cells, NK cells, monocytes, and some other immune cells. It also induces regulatory T cells and anti-inflammatory macrophages. HLA-G is important e.g. for maternal tolerance to the fetus, and for immunomodulation in particular adult tissues, such as in cornea, pancreatic islets, thymus and other. On the other hand, it is expressed in many solid and hematologic malignancies, where it contributes to evasion of the immune surveillance. HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome. Unlike most other MHC glycoproteins, HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule. It concerns both transmembrane and soluble HLA-G isoforms. Among other, HLA-G can promote Th2 immunological response and downregulate Th1 immunological response. For its benefits regarding allograft tolerance, including embryo implantation, soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization. Similarly, sHLA-G concentrations in maternal serum are decreased in preeclampsia. Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance. On the other hand, increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells. Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies, which can weaken the therapy effect. Monitoring of HLA-G in patients thus has a wide usage.
    ID gène
    3135
    UniProt
    P17693
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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