NEFH anticorps
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- Antigène Voir toutes NEFH Anticorps
- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
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Reactivité
- Mammifères
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp NEFH est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Specificité
- The antibody NF-01 recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa intracellular antigen) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
- Réactivité croisée (Details)
- Mammalian
- Purification
- Purified by protein-A affinity chromatography.
- Pureté
- > 95 % (by SDS-PAGE)
- Immunogène
- Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
- Clone
- NF-01
- Isotype
- IgG1
- Top Product
- Discover our top product NEFH Anticorps primaire
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- Indications d'application
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Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL. - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Do not freeze.
- Stock
- 4 °C
- Stockage commentaire
- Store at 2-8°C. Do not freeze.
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Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord." dans: Histochemistry, Vol. 100, Issue 6, pp. 495-502, (1994) (PubMed).
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Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord." dans: Histochemistry, Vol. 100, Issue 6, pp. 495-502, (1994) (PubMed).
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- Antigène
- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
- Autre désignation
- Neurofilament heavy protein (NEFH Produits)
- Synonymes
- anticorps NF-H, anticorps NF200, anticorps Nfh, anticorps mKIAA0845, anticorps NFH, anticorps NEFH, anticorps neurofilament heavy polypeptide, anticorps neurofilament, heavy polypeptide, anticorps neurofilament heavy, anticorps MCYG_06231, anticorps Nefh, anticorps NEFH
- Sujet
- Neurofilament heavy,Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights –, neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.,NFH, NEFH, Neurofilament heavy chain, CMT2CC
- ID gène
- 4744
- UniProt
- P12036
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