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Acrosin anticorps

ACR Reactivité: Porc WB, FACS, ICC Hôte: Souris Monoclonal ACR-2 unconjugated
N° du produit ABIN94455
  • Antigène Voir toutes Acrosin (ACR) Anticorps
    Acrosin (ACR)
    Reactivité
    • 38
    • 17
    • 17
    • 16
    • 2
    • 2
    • 2
    • 1
    Porc
    Hôte
    • 38
    • 2
    • 1
    Souris
    Clonalité
    • 39
    • 2
    Monoclonal
    Conjugué
    • 20
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Acrosin est non-conjugé
    Application
    • 34
    • 18
    • 13
    • 13
    • 12
    • 8
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
    Specificité
    The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
    Aucune reactivité croisée
    Boeuf (Vache), Chien, Humain
    Réactivité croisée (Details)
    Porcine
    Purification
    Purified by protein-A affinity chromatography.
    Pureté
    > 95 % (by SDS-PAGE)
    Immunogène
    Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
    Clone
    ACR-2
    Isotype
    IgG1
    Top Product
    Discover our top product ACR Anticorps primaire
  • Indications d'application
    Flow cytometry: Recommended dilution: 1-12 μg/mL. Intracellular staining.
    Immunocytochemistry: Membrane permeabilization (acetone) is essential.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Do not freeze.
  • Ded, Dostalova, Zatecka, Dorosh, Komrskova, Peknicova: "Fluorescent analysis of boar sperm capacitation process in vitro." dans: Reproductive biology and endocrinology : RB&E, Vol. 17, Issue 1, pp. 109, (2020) (PubMed).

    Ded, Dostalova, Dorosh, Dvorakova-Hortova, Peknicova: "Effect of estrogens on boar sperm capacitation in vitro." dans: Reproductive biology and endocrinology : RB&E, Vol. 8, pp. 87, (2010) (PubMed).

    Peknicova, Capkova, Geussova, Ivanova, Mollova: "Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro." dans: Theriogenology, Vol. 56, Issue 2, pp. 211-23, (2001) (PubMed).

    Moos, Peknicova, Tesarik: "Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction." dans: Biology of reproduction, Vol. 49, Issue 2, pp. 408-15, (1993) (PubMed).

    Moos, Peknicova, Tesarik: "Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction." dans: Biochimica et biophysica acta, Vol. 1176, Issue 3, pp. 199-207, (1993) (PubMed).

    Moos, Tesarik, Leca, Peknicova: "Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin." dans: FEBS letters, Vol. 294, Issue 1-2, pp. 27-30, (1992) (PubMed).

    Peknicová, Moos: "Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test." dans: Andrologia, Vol. 22, Issue 5, pp. 427-35, (1991) (PubMed).

  • Antigène
    Acrosin (ACR)
    Autre désignation
    Acrosin (ACR Produits)
    Synonymes
    anticorps ACR, anticorps MGC130972, anticorps Acro, anticorps Acrosin, anticorps acrosin, anticorps acrosin L homeolog, anticorps acrosin prepropeptide, anticorps ACR, anticorps acr.L, anticorps Acr
    Sujet
    Acrosin,Acrosin is a serine proteinase expressed in the acrosome of mature spermatozoa. This enzyme facilitates penetration of the sperm through the zona pellucida of the ovum.,ACR, FBP53
    ID gène
    397098
    UniProt
    P08001
    Pathways
    cAMP Metabolic Process
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