SIT1 anticorps

N° du produit ABIN94465

Détail du produit anti-SIT1 anticorps

Antigène: SIT1 (Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SIT1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

Specificité: The antibody SIT-01 reacts with an intracellular epitope of SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with murine SIT.

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Bacterially produced recombinant intracellular fragment of human SIT.

Clone: SIT-01

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1-5 μg/mL, intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-SIT1 (ABIN94465)

Tedoldi, Paterson, Hansmann, Natkunam, Rüdiger, Angelisova, Du, Roberton, Roncador, Sanchez, Pozzobon, Masir, Barry, Pileri, Mason, Marafioti, Horejsí: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." dans: Blood, Vol. 107, Issue 1, pp. 213-21, (2005) (PubMed).

Horejsí, Zhang, Schraven: "Transmembrane adaptor proteins: organizers of immunoreceptor signalling." dans: Nature reviews. Immunology, Vol. 4, Issue 8, pp. 603-16, (2004) (PubMed).

Détails sur SIT1

Antigène: SIT1 (Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1))

Autre désignation: SIT (SIT1 Produits)

Synonymes: anticorps SIT1, anticorps RP11-331F9.5, anticorps SIT, anticorps SIT-R, anticorps Sit, anticorps signaling threshold regulating transmembrane adaptor 1, anticorps suppression inducing transmembrane adaptor 1, anticorps SIT1, anticorps Sit1

Sujet: Signaling threshold regulating transmembrane adapt,SIT is a 21 kDa transmembrane adaptor protein expressed exclusively in lymphoid organs. It acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.,SIT1

ID gène: 27240

UniProt: Q9Y3P8