PAG1 anticorps (AA 97-432)

N° du produit ABIN94497

Cet anticorps anti-PAG1 Polyclonal Lapin (ABIN94497) détecte spécifiquement PAG1 dans WB, IP et IHC (p). L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour PAG1 anticorps (AA 97-432) (ABIN94497)

Antigène: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PAG1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PAG1 anticorps

Épitope: AA 97-432

Fonction: Anti-PAG1 Purified

Réactivité croisée (Details): Mouse, Human

Attributs du produit: The polyclonal antibody recognizes intracellular part of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).

Information d'application

Indications d'application: Immunohistochemistry (paraffin sections): Positive tissue: tonsil, colon germinal center.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Détails sur PAG1

Antigène: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Autre désignation: PAG1

Sujet: Phosphoprotein membrane anchor with glycosphingoli,PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.,CBP, PAG

ID gène: 94212

UniProt: Q3U1F9

Pathways: Signalisation p53, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events