LIME anticorps (AA 141-295)

N° du produit ABIN94499

Détail du produit anti-LIME anticorps

Antigène: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Épitope: AA 141-295

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIME est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: The polyclonal antibody reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.

Aucune reactivité croisée: Souris

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-LIME (ABIN94499)

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." dans: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).

Détails sur LIME

Antigène: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Autre désignation: LIME (LIME1 Produits)

Synonymes: anticorps LIME1, anticorps DKFZp459A158, anticorps LIME, anticorps LP8067, anticorps dJ583P15.4, anticorps 2810038K19Rik, anticorps RGD1562795, anticorps Lck interacting transmembrane adaptor 1, anticorps LIME1, anticorps Lime1

Sujet: Lck interacting transmembrane adaptor 1,LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.,LITA1

ID gène: 54923

UniProt: Q9H400