Alkaline Phosphatase anticorps

N° du produit ABIN94943

Détail du produit anti-Alkaline Phosphatase anticorps

Antigène: Alkaline Phosphatase (ALP)

Reactivité: E. coli

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Alkaline Phosphatase est non-conjugé

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined. Some minimal crossreactivity has been reported when lysates of cell extracts overproducing E.coli proteins are probed.

Pureté: Anti-ALKALINE PHOSPHATASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Alkaline Phosphatase [E.coli]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: ALKALINE PHOSPHATASE Antibody has been assayed against 1.0 µg of Alkaline Phosphatase [E.coli] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:25.000 to 1:125.000 of the reconstitution concentration is suggested for this product.  A 1:10.000 dilution has been reported to be useful for the detection by immunoblot of an integral membrane protein from a gram-positive organism expressed as a LacZ/PhoA chimeric derivative in E.coli.  The researcher should determine optimal titers for other applications.
ELISA Dilution: 1:4.000 - 1:20.000
Western Blot Dilution: 1:500 - 1:2.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur Alkaline Phosphatase

Antigène: Alkaline Phosphatase (ALP)

Autre désignation: Alkaline Phosphatase (ALP Produits)

Synonymes: anticorps ALP, anticorps ALP1, anticorps CRASH, anticorps ECK0378, anticorps JW0374, anticorps psiA, anticorps 2410004D18Rik, anticorps AU040643, anticorps AW060375, anticorps ILC, anticorps CTACK, anticorps ESkine, anticorps Akp2, anticorps alp, anticorps zgc:56672, anticorps DDBDRAFT_0205491, anticorps DDBDRAFT_0231570, anticorps DDB_0205491, anticorps DDB_0231570, anticorps Actn2lp, anticorps Alp, anticorps asparaginase like 1, anticorps bacterial alkaline phosphatase, anticorps C-C motif chemokine ligand 27, anticorps alkaline phosphatase, liver/bone/kidney, anticorps alkaline phosphatase, anticorps PDZ and LIM domain 3, anticorps ASRGL1, anticorps phoA, anticorps Asrgl1, anticorps CCL27, anticorps alpl, anticorps alp, anticorps Pdlim3

Sujet: Synonyms: Akp2 antibody, ALP antibody, Nagao isozyme antibody, Placental alkaline phosphatase antibody, Placental like alkaline phosphatase antibody, PLAP 1 antibody, PLAP like antibody, Regan isozyme antibody, Kasahara isozyme antibody

ID gène: 945041

UniProt: P00634