PUMA anticorps (AA 2-16)
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- Antigène Voir toutes PUMA (BBC3) Anticorps
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
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Épitope
- AA 2-16
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Reactivité
- Humain, Rat, Souris
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PUMA est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Recognizes the p53 - upregulated modulator of apoptosis, also known as Bcl-2 binding component 3, JFY-1 or Bcl-2. PUMA is a 193 amino acid ~20 kD pro-apoptotic member of the BCL-2 family. Bcl-2 binding component 3 is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53. Four isoforms of PUMA have been described due to alternative splicing. Is expected to recognize the canonical isofom 1, also known as PUMA alpha, it is not expected to recognize any of the other isoforms of PUMA.
- Homologie
- Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Porcine (100%) Opossum (87%).
- Purification
- Purified
- Immunogène
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Synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human PUMA alpha. Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Porcine (100%), Opossum (87%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product BBC3 Anticorps primaire
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- Indications d'application
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Approved: IHC, IHC-P (2.5 μg/mL), WB (2 - 4 μg/mL)
Usage: Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Histology Positive Control Tissue: Human breast carcinoma. Western Blot: detects a band of approximately 23kD in K562 cell lysates. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 0.02 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- avoid freeze thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Antigène
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
- Autre désignation
- PUMA (BBC3 Produits)
- Synonymes
- anticorps BBC3, anticorps Puma, anticorps JFY-1, anticorps JFY1, anticorps PUMA, anticorps PUMA/JFY1, anticorps BCL2 binding component 3, anticorps Bcl-2 binding component 3, anticorps BBC3, anticorps Bbc3, anticorps bbc3
- Sujet
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Name/Gene ID: BBC3
Family: Apoptosis
Synonyms: BBC3, Bcl-2-binding component 3, BCL2 binding component 3, JFY-1, JFY1, PUMA - ID gène
- 27113
- UniProt
- Q9BXH1
- Pathways
- Signalisation p53, Positive Regulation of Endopeptidase Activity
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