Strep Tag II anticorps (Biotin)

N° du produit ABIN962829

Détail du produit

Antigène: Strep Tag II

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Biotin

Application: Western Blotting (WB), ELISA

Marque: THE™

Séquence: NWSHPQFEK

Specificité: THE^TM NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.

Attributs du produit: The THE^TM NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse is THE^TM NWSHPQFEK Tag Antibody conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of NWSHPQFEK (Strep II tag) tagged fusion proteins.

Purification: Protein A affinity column

Immunogène: NWSHPQFEK peptide conjugated - KLH

Clone: 5A9F9

Isotype: IgG1

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/mL
ELISA: 0.001-0.002 µg/mL

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.

Buffer: PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

Détail du antigène

Antigène: Strep Tag II

Classe de substances: Tag

Sujet: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep II tag) is a nine amino acid peptide with high specificity and affinity towards streptavidin.