V5 Epitope Tag anticorps (Biotin)
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- Antigène Tous les produits V5 Epitope Tag
- V5 Epitope Tag
- Reactivité
- Veuillez nous consulter SVP
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp V5 Epitope Tag est conjugé à/à la Biotin
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Application
- Western Blotting (WB), ELISA
- Marque
- THE™
- Séquence
- GKPIPNPLLG LDST
- Specificité
- THETM V5 Tag Antibody [Biotin], mAb, Mouse recognizes N-terminal and C-terminal V5 tagged fusion proteins.
- Attributs du produit
- The THETM V5 Tag Antibody [Biotin], mAb, Mouse is THETM V5 Tag Antibody conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of V5 Tagged fusion proteins.
- Purification
- Protein A affinity column
- Immunogène
- V5 tag peptide conjugated - KLH
- Clone
- 4C12E11
- Isotype
- IgG2a
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- Indications d'application
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.005-0.05 µg/mL
Western blot: 0.2-1 µg/mL - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
- Buffer
- PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % Sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing cycles.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
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- Antigène
- V5 Epitope Tag
- Autre désignation
- V5 Tag (V5 Epitope Tag Produits)
- Classe de substances
- Tag
- Sujet
- V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
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