TUBA1B anticorps (C-Term)

N° du produit ABIN964563

N° du produit (Fournisseur): 200-301-880

Détail du produit anti-TUBA1B anticorps

Antigène: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Épitope: C-Term

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TUBA1B est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)

N° du produit (Fournisseur): 200-301-880

Fournisseur: Rockland

Fonction: Alpha-Tubulin Antibody

Réactivité croisée (Details): This Loading Control antibody is directed against alpha tubulin.

Attributs du produit: Synonyms: mouse anti-Alpha-Tubulin Antibody, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control

Purification: Anti-Tubulin Loading Control Antibody was purified by Protein A chromatography.

Stérilité: Sterile filtered

Immunogène: Optional[Immunogen]: Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.

Clone: 17H11-F10

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 2.5 μg/mL

Application Note: Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.

Western Blot Dilution: 1:1,000

ELISA Dilution: 1:300,000

IF Microscopy Dilution: 0.1 μg/mL

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Anti-Tubulin Antibody at -20° C prior to opening. Aliquot Loading Control Antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge Tubulin Loading Control Antibody if not completely clear after standing at room temperature. This Anti-Tubulin Loading Control Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-TUBA1B (ABIN964563)

Clayton, Ban, Liu, Serra: "Canonical and noncanonical TGF-β signaling regulate fibrous tissue differentiation in the axial skeleton." dans: Scientific reports, Vol. 10, Issue 1, pp. 21364, (2021) (PubMed).

Chavez, Coricor, Perez, Seo, Serra: "SOX9 protein is stabilized by TGF-β and regulates PAPSS2 mRNA expression in chondrocytes." dans: Osteoarthritis and cartilage, Vol. 25, Issue 2, pp. 332-340, (2018) (PubMed).

Patel, Blaauboer, Tucker, Mansouri, Ruiz-Moreno, Hamann, Schumann, Opitz, Jin: "The Common R71H-G230A-R293Q Human TMEM173 Is a Null Allele." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 2, pp. 776-787, (2017) (PubMed).

Roy, Jerman, Jozsef, McNamara, Onyekaba, Sun, Marin: "Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation." dans: The Journal of biological chemistry, Vol. 292, Issue 43, pp. 17703-17717, (2017) (PubMed).

Laitem, Zaborowska, Isa, Kufs, Dienstbier, Murphy: "CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes." dans: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 396-403, (2015) (PubMed).

Blaauboer, Gabrielle, Jin: "MPYS/STING-mediated TNF-α, not type I IFN, is essential for the mucosal adjuvant activity of (3'-5')-cyclic-di-guanosine-monophosphate in vivo." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 1, pp. 492-502, (2014) (PubMed).

Détails sur TUBA1B

Antigène: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Autre désignation: TUBA1B (TUBA1B Produits)

Sujet: Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control.

ID gène: 17986283

UniProt: P68363

Pathways: Dynamique des Microtubules, M Phase