ZNF148 anticorps

N° du produit ABIN964672

Détail du produit anti-ZNF148 anticorps

Antigène: ZNF148 (Zinc Finger Protein 148 (ZNF148))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ZNF148 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Specificité: This product was prepared from monospecific antiserum by delipidation and defibrination. This polyclonal antibody is specific for human ZBP-89.  Reactivity with ZBP-89 from other species has not been determined.

Attributs du produit: The GI tract abundantly expresses growth factors many of which bind and activate the EGF receptor present on mucosal cells. One such factor is the zinc finger protein (ZBP-89) that binds to a GC-rich DNA element in the gastrin promoter and confers EGF responsiveness. The full-length protein functions as a repressor of growth factor signals regulating the gastrin promoter. Several other growth related promoters are also regulated by ZBP-89. ZBP-89 is one of a family of related transcriptional regulators. It has been reported in recent studies that ZBP-89 regulates growth in part by stimulating the cyclin-dependent kinase inhibitor, p21waf1, in a butyrate-dependent manner through recruitment of the histone acetyl transferase p300. Moreover, ZBP-89 triggers growth arrest in a p53-dependent manner by preventing nuclear export of p53. ZBP-89 also induces apoptosis, but this process occurs independent of p53.

Stérilité: Sterile filtered

Immunogène: Purified full length ZBP-89 recombinant protein expressed in E.coli.
Immunogen Type: RecombinantProtein

Information d'application

Indications d'application: This polyclonal antibody reacts human ZBP-89 in a variety of immunological assays including western blot and ELISA.  Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For immunoblotting a 1:5,000 dilution is recommended. A band at approximately 89 kDa corresponding to human ZBP-89 is detected. Human monocytes or macrophages or nuclear extracts from PMA treated U937 cells can be used as a positive control. For ELISA a 1:10,000 to 1:30,000 dilution is recommended. Researchers should determine optimal titers for other applications.

Commentaires:

Gene Name: ZBP-89

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 90 mg/mL

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Références pour anticorps anti-ZNF148 (ABIN964672)

Ohneda, Ohmori, Ishijima, Nakano, Yamamoto: "Characterization of a functional ZBP-89 binding site that mediates Gata1 gene expression during hematopoietic development." dans: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30187-99, (2009) (PubMed).

Guyot, Murai, Fujiwara, Valverde-Garduno, Hammett, Wells, Dear, Orkin, Porcher, Vyas: "Characterization of a megakaryocyte-specific enhancer of the key hemopoietic transcription factor GATA1." dans: The Journal of biological chemistry, Vol. 281, Issue 19, pp. 13733-42, (2006) (PubMed).

Détails sur ZNF148

Antigène: ZNF148 (Zinc Finger Protein 148 (ZNF148))

Autre désignation: ZBP-89 (ZNF148 Produits)

Synonymes: anticorps BERF-1, anticorps BFCOL1, anticorps HT-BETA, anticorps ZBP-89, anticorps ZFP148, anticorps pHZ-52, anticorps 2210405J08Rik, anticorps AI480666, anticorps AW045217, anticorps Znf148, anticorps Zbp-89, anticorps fb06h05, anticorps wu:fb06h05, anticorps zfp149, anticorps zgc:165342, anticorps SLC12A8, anticorps zinc finger protein 148, anticorps ZNF148, anticorps Zfp148, anticorps znf148

Sujet: The GI tract abundantly expresses growth factors many of which bind and activate the EGF receptor present on mucosal cells. One such factor is the zinc finger protein (ZBP-89) that binds to a GC-rich DNA element in the gastrin promoter and confers EGF responsiveness. The full-length protein functions as a repressor of growth factor signals regulating the gastrin promoter. Several other growth related promoters are also regulated by ZBP-89. ZBP-89 is one of a family of related transcriptional regulators. It has been reported in recent studies that ZBP-89 regulates growth in part by stimulating the cyclin-dependent kinase inhibitor, p21waf1, in a butyrate-dependent manner through recruitment of the histone acetyl transferase p300. Moreover, ZBP-89 triggers growth arrest in a p53-dependent manner by preventing nuclear export of p53. ZBP-89 also induces apoptosis, but this process occurs independent of p53.
Synonyms: Transcription factor ZBP89 antibody, Zinc finger DNA binding protein 89 antibody, Zinc finger protein 148 antibody, ZNF 148 antibody

ID gène: 256711

UniProt: Q9UQR1