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NF-kB p65 anticorps (C-Term)

Name: NF-kB p65 anticorps (C-Term)
Brand: antibodies-online
SKU: ABIN964680
Price: 623.7 EUR
Availability: InStock

NFkBP65 Reactivité: Humain WB, IHC, ELISA, IF, FM Hôte: Souris Monoclonal 27F9-G4 unconjugated

anti NFKB p65 (Rel A) monoclonal antibody was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. (NF-kB p65 anticorps (C-Term))

Antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. (NF-kB p65 anticorps (C-Term))

IHC

Anti NFkB monoclonal antibody – Immunocytochemistry Tissue: Human Fibroblasts Top: Before activation Bottom: After activation with poly IC Left: 600-401-271 anti p65 NLS specific lot 18372 Right: Monoclonal antibody C-Term The two antibodies that are shown target different regions of the p65 protein. (NF-kB p65 anticorps (C-Term))

Immunofluorescence (IF) image for anti-Nuclear Factor-kB p65 (NFkBP65) (C-Term) antibody (ABIN964680) (NF-kB p65 anticorps (C-Term))

Monoclonal anti NFKB p65 (Rel A) antibody was used to detect p65 in b. (NF-kB p65 anticorps (C-Term))

monoclonal anti p65 was used to detect p65 by Western blot. (NF-kB p65 anticorps (C-Term))

6 images

(2 références)

N° du produit ABIN964680

Fiche de données

Antigène Voir toutes NF-kB p65 (NFkBP65) Anticorps

NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
Épitope
37

31

28

26

23

22

22

19

19

16

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13

9

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6

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C-Term
Reactivité
501

352

286

41

35

28

24

21

19

16

14

11

5

2

1

1

1

1
Humain
Hôte
453

50

3

2

1
Souris
Anticorps secondaires appropriés
Clonalité
435

74
Monoclonal
Conjugué
267

25

19

14

12

10

10

10

10

10

10

10

10

10

10

10

10

9

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8

8

8

6

2

2

1
Cet anticorp NF-kB p65 est non-conjugé
Application
394

176

131

119

104

102

85

71

70

28

25

9

7

7

7

6

4

4

3

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Fonction

NFkB p65 Antibody

Réactivité croisée (Details)

Reactivity was confirmed by ELISA against peptide conjugated carrier protein and by Western blot against HeLa whole cell lysate.

Attributs du produit

Synonyms: mouse anti-NF-kB p65 Antibody, mouse anti-Rel A antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Nuclear factor NF-kappa-B p65 subunit

Purification

Anti-NF-kB p65 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography and showed a single band by IEP (immunoelectrophoresis) when tested with anti-mouse antibody.

Stérilité

Sterile filtered

Immunogène

Immunogen: NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide

Clone

27F9-G4

Isotype

IgG2a kappa

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anti-Nuclear Factor-kB p65 (NFkBP65) (AA 10-37), (N-Term) antibody
NFkBP65 Reactivité: Humain, Souris WB, IF, IHC (p), FACS Hôte: Lapin Polyclonal RB20786 unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pThr435) antibody
NFkBP65 Reactivité: Humain, Souris, Rat WB, IHC, ELISA, IP, IF, ICC Hôte: Lapin Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pSer281) antibody
NFkBP65 Reactivité: Humain, Souris, Rat WB, IHC, ELISA, IF, ICC Hôte: Lapin Polyclonal unconjugated

Indications d'application

Immunohistochemistry Dilution: 1:200-1:600
Application Note: Anti-NF-kB p65 Antibody is a mouse monoclonal antibody directed against NFkB p65 (Rel A) and recognizes a 65 kD band by Western blot against HeLa whole cell lysate. Control peptide (100-4165p) is sold separately. This product tested in WB, ICC, IHC, and IF.
Western Blot Dilution: 1:1,000-1:5,000
ELISA Dilution: 1:50,000-1:100,000
IF Microscopy Dilution: 1:5,000
Other: User Optimized

Restrictions

For Research Use only
Format

Liquid

Concentration

1.0 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Store NF-kB p65 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption

12 months
Sikorski, Mehta, Inngjerdingen, Thakor, Kling, Kalina, Nyman, Stensland, Zhou, de Souza, Holden, Stuchly, Templin, Lund-Johansen: "A high-throughput pipeline for validation of antibodies." dans: Nature methods, Vol. 15, Issue 11, pp. 909-912, (2019) (PubMed).

Merkhofer, Cogswell, Baldwin: "Her2 activates NF-kappaB and induces invasion through the canonical pathway involving IKKalpha." dans: Oncogene, Vol. 29, Issue 8, pp. 1238-48, (2010) (PubMed).
Antigène

NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Autre désignation

RELA (NFkBP65 Produits)

Sujet

Background: NFkappaB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaBalpha. Cell Biology, Nuclear Signaling, Neuroscience and Signal Transduction Research.

ID gène

5970, 223468676

UniProt

Q04206

Pathways

Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins