NEU1 anticorps (Internal Region)

N° du produit ABIN964880

Détail du produit anti-NEU1 anticorps

Antigène: NEU1 (Sialidase 1 (Lysosomal Sialidase) (NEU1))

Épitope: Internal Region

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NEU1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Specificité: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.  This antibody reacts with human Neu2.  Based on sequence we expect this antibody to react with neuraminidase from other sources, although specific reactivity has not been confirmed.  Cross-reactivity against Neu1 has not yet been established.  Neuraminidases are highly conserved in mammals and therefore cross reactivity is expected with mouse and rat Neu2.

Attributs du produit: Neuraminidases or sialidases are exoglycosidases that catalyze the cleavage of alpha-glycosidically linked terminal N-acetyl neuraminic acid from sialylated glycoconjugates. They are widely spread in nature, occurring in viruses, bacteria, fungi, protozoa, birds and mammals. Together, the neuraminidases form a family of hydrolases that share a conserved active site and similar sequence motifs. Three types of neuraminidase are found in mammals and are defined as lysosomal, plasma membrane and cytosolic on the basis of their biochemical properties and subcellular distribution. Lysosomal N-acetyl-alpha-neuraminidase (NEU1) has significant primary structure characteristics of other mammalian and microbial sialidases with similar substrate specificity. However, unlike other members of this family, lysosomal neuraminidase requires the carboxypeptidase protective protein/cathepsin A (PPCA) for intracellular transport and lysosomal activation. The enzyme is only catalytically active when it is bound to PPCA and is a component of a high molecular weight, multi-protein complex containing PPCA, ß-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase. Using a hamster Sial3 probe, Monti et al. (1999) identified the gene encoding sialidase-2, which they designated NEU2, from a human genomic library. The 2 putative exons of NEU2 encode a deduced 380-amino acid protein with a calculated molecular mass of 42.23 kD. The NEU2 protein has significant homology with the mammalian, viral, and bacterial sialidases. It shares over 72% similarity with the hamster and rat cytosolic sialidases and over 42% similarity with human NEU1. NEU2 contains a potential N-linked glycosylation site, 2 aspartic acid block consensus sequences, and an N-terminal F/YRIP sequence motif which is part of the active site of other sialidase enzymes. Monti et al. hypothesized that NEU2 has a cytosolic localization because it does not contain a cleavage site, transmembrane domain, or targeting motifs.

Stérilité: Sterile filtered

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 110-124 of Human Neu2.
Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application: This antibody is suitable for western blotting, immunocytochemistry, immunoprecipitation, transfected cell culture, primary cell culture, and ELISA. A single band of the expected apparent molecular weight (43 kDa) was observed at a 1:500 dilution incubated for 1 h at room temperature. A second lower molecular weight band may represent a truncated form of this protein. Neuraminidase is not very abundant in most tissues and its detection using this antibody may require further optimization. Researchers should determine optimal titers for other applications.

Commentaires:

Gene Name: NEU2

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.9 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Détails sur NEU1

Antigène: NEU1 (Sialidase 1 (Lysosomal Sialidase) (NEU1))

Autre désignation: Neuraminidase (NEU1 Produits)

Synonymes: anticorps NANH, anticorps NEU, anticorps SIAL1, anticorps MGC81958, anticorps AA407268, anticorps AA407316, anticorps Aglp, anticorps Apl, anticorps Bat-7, anticorps Bat7, anticorps G9, anticorps Map-2, anticorps Neu, anticorps Neu-1, anticorps neuraminidase 1, anticorps neuraminidase 1 (lysosomal sialidase) L homeolog, anticorps NEU1, anticorps neu1.L, anticorps neu1, anticorps Neu1

Classe de substances: Influenza Protein

Sujet: Neuraminidases or sialidases are exoglycosidases that catalyze the cleavage of α-glycosidically linked terminal N-acetyl neuraminic acid from sialylated glycoconjugates. They are widely spread in nature, occurring in viruses, bacteria, fungi, protozoa, birds and mammals. Together, the neuraminidases form a family of hydrolases that share a conserved active site and similar sequence motifs. Three types of neuraminidase are found in mammals and are defined as lysosomal, plasma membrane and cytosolic on the basis of their biochemical properties and subcellular distribution. Lysosomal N-acetyl-α-neuraminidase (NEU1) has significant primary structure characteristics of other mammalian and microbial sialidases with similar substrate specificity. However, unlike other members of this family, lysosomal neuraminidase requires the carboxypeptidase protective protein/cathepsin A (PPCA) for intracellular transport and lysosomal activation. The enzyme is only catalytically active when it is bound to PPCA and is a component of a high molecular weight, multi-protein complex containing PPCA, ß-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase. Using a hamster Sial3 probe, Monti et al. (1999) identified the gene encoding sialidase-2, which they designated NEU2, from a human genomic library. The 2 putative exons of NEU2 encode a deduced 380-amino acid protein with a calculated molecular mass of 42.23 kD. The NEU2 protein has significant homology with the mammalian, viral, and bacterial sialidases. It shares over 72% similarity with the hamster and rat cytosolic sialidases and over 42% similarity with human NEU1. NEU2 contains a potential N-linked glycosylation site, 2 aspartic acid block consensus sequences, and an N-terminal F/YRIP sequence motif which is part of the active site of other sialidase enzymes. Monti et al. hypothesized that NEU2 has a cytosolic localization because it does not contain a cleavage site, transmembrane domain, or targeting motifs.
Synonyms: Acetylneuraminyl hydrolase antibody, G9 sialidase antibody, Lysosomal sialidase antibody, N acetyl alpha neuraminidase 1 antibody, NANH antibody, Neu 1 antibody

ID gène: 4759, 222352169

UniProt: Q9Y3R4

Pathways: SARS-CoV-2 Protein Interactome