POLB anticorps (C-Term)

N° du produit ABIN964928

N° du produit (Fournisseur): 600-401-c65

Détail du produit anti-POLB anticorps

Antigène: POLB (Polymerase (DNA Directed), beta (POLB))

Épitope: C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLB est non-conjugé

Application: Western Blotting (WB), ELISA

N° du produit (Fournisseur): 600-401-c65

Fournisseur: Rockland

Fonction: POLB Antibody

Réactivité croisée (Details): This antibody reacts with overexpressed and endogenous POLß protein.

Attributs du produit: Synonyms: rabbit anti-POLB antibody, rabbit anti-DNA polymerase beta antibody, DNA polymerase ß, POLß, POL-B, POL-ß, pol-beta, beta-pol

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of the POLß protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified POLß antibody has been tested by ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38.1 kDa in size corresponding to POLß by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 0.5 μg/mL

ELISA Dilution: 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.4 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-POLB (ABIN964928)

Asagoshi, Liu, Masaoka, Lan, Prasad, Horton, Brown, Wang, Bdour, Sobol, Taylor, Yasui, Wilson: "DNA polymerase beta-dependent long patch base excision repair in living cells." dans: DNA repair, Vol. 9, Issue 2, pp. 109-19, (2010) (PubMed).

Sobol, Foley, Nyska, Davidson, Wilson: "Regulated over-expression of DNA polymerase beta mediates early onset cataract in mice." dans: DNA repair, Vol. 2, Issue 5, pp. 609-22, (2004) (PubMed).

Détails sur POLB

Antigène: POLB (Polymerase (DNA Directed), beta (POLB))

Autre désignation: POLB (POLB Produits)

Sujet: Background: The POLß protein belongs to the DNA polymerase type-X family. It is a DNA polymerase involved in gap-filling DNA synthesis or base excision and repair in a stepwise distributive fashion unlike other DNA polymerases. It is required for DNA maintenance, replication, recombination, and drug resistance. It is normally found as a monomer, in the cytoplasm and it translocates to the nucleus upon DNA damage.

ID gène: 5423

NCBI Accession: NP_002681

UniProt: P06746

Pathways: Réparation de l'ADN