Western Blot: 1: 500- 1: 1,000 IHC(P): 1: 500- 1: 1,000 ELISA: Propose dilution 1: 10,000. Determining optimal working dilutions by titration test.
Restrictions
For Research Use only
Stock
-20 °C
Fadden, Haystead, Lawrence: "Identification of phosphorylation sites in the translational regulator, PHAS-I, that are controlled by insulin and rapamycin in rat adipocytes." dans: The Journal of biological chemistry, Vol. 272, Issue 15, pp. 10240-7, (1997) (PubMed).
Pause, Belsham, Gingras, Donzé, Lin, Lawrence, Sonenberg: "Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function." dans: Nature, Vol. 371, Issue 6500, pp. 762-7, (1994) (PubMed).
Antigène
eIF4EBP1 (EIF4EBP1)
(Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.