Western Bloting: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. ELISA: Propose dilution 1/10000. Not yet tested in other applications. Determining optimal working dilutions by titration test.
Restrictions
For Research Use only
Format
Liquid
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Llinas, Stura, Ménez, Kiss, Stigbrand, Millán, Le Du: "Structural studies of human placental alkaline phosphatase in complex with functional ligands." dans: Journal of molecular biology, Vol. 350, Issue 3, pp. 441-51, (2005) (PubMed).
Chen, Anderson, Hiroi, Medof: "Proprotein interaction with the GPI transamidase." dans: Journal of cellular biochemistry, Vol. 88, Issue 5, pp. 1025-37, (2003) (PubMed).
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placentallike form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. Synonyms: ALP, PALP, PLAP, FLJ61142, ALPP