CDK2 (Ab-160) antibody detects endogenous levels of total CDK2 protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogène
The antiserum was produced against synthesized non-phosphopeptide derived from human CDK2 around the phosphorylation site of threonine160 (T-Y-TP-H-E).
CDK2
Reactivité: Humain
WB, PLA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1:500~1:1000.
Restrictions
For Research Use only
Format
Liquid
Concentration
100ug/100ul.
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
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Ukomadu, Dutta: "Inhibition of cdk2 activating phosphorylation by mevastatin." dans: The Journal of biological chemistry, Vol. 278, Issue 7, pp. 4840-6, (2003) (PubMed).
Morris, Gondeau, Tainer, Divita: "Kinetic mechanism of activation of the Cdk2/cyclin A complex. Key role of the C-lobe of the Cdk." dans: The Journal of biological chemistry, Vol. 277, Issue 26, pp. 23847-53, (2002) (PubMed).
Brown, Noble, Lawrie, Morris, Tunnah, Divita, Johnson, Endicott: "Effects of phosphorylation of threonine 160 on cyclin-dependent kinase 2 structure and activity." dans: The Journal of biological chemistry, Vol. 274, Issue 13, pp. 8746-56, (1999) (PubMed).