MEK2 (Ab-394) antibody detects endogenous levels of total MEK2 protein
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogène
The antiserum was produced against synthesized non-phosphopeptide derived from human MEK2 around the phosphorylation site of threonine 394 (P-G-TP-P-T).
MAP2K1
Reactivité: Humain
WB, IHC, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1:500~1:1000 IHC: 1:50~1:100.
Restrictions
For Research Use only
Format
Liquid
Concentration
100ug/100ul.
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Alessi, Saito, Campbell, Cohen, Sithanandam, Rapp, Ashworth, Marshall, Cowley: "Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1." dans: The EMBO journal, Vol. 13, Issue 7, pp. 1610-9, (1994) (PubMed).
Rosen, Ginty, Weber, Greenberg: "Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras." dans: Neuron, Vol. 12, Issue 6, pp. 1207-21, (1994) (PubMed).
Cowley, Paterson, Kemp, Marshall: "Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells." dans: Cell, Vol. 77, Issue 6, pp. 841-52, (1994) (PubMed).
Antigène
MEK1 (MAP2K1)
(Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))