P27Kip1 (Ab-10) antibody detects endogenous levels of total p27Kip1 protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogène
The antiserum was produced against synthesized non-phosphopeptide derived from human p27Kip1 around the phosphorylation site of serine 10 (N-G-SP-P-S).
CDKN1B
Reactivité: Humain, Souris, Rat
WB, ELISA, IHC, IF, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1:500~1:1000 IHC: 1:50~1:100.
Restrictions
For Research Use only
Format
Liquid
Concentration
100ug/100ul.
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Le, Claret, Lammayot, Tian, Deshpande, LaPushin, Tari, Bast: "The role of cyclin-dependent kinase inhibitor p27Kip1 in anti-HER2 antibody-induced G1 cell cycle arrest and tumor growth inhibition." dans: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 23441-50, (2003) (PubMed).
Connor, Kotchetkov, Cariou, Resch, Lupetti, Beniston, Melchior, Hengst, Slingerland: "CRM1/Ran-mediated nuclear export of p27(Kip1) involves a nuclear export signal and links p27 export and proteolysis." dans: Molecular biology of the cell, Vol. 14, Issue 1, pp. 201-13, (2003) (PubMed).
Chopra, Fernandez De Mattos, Lam, Mann: "Jab1 co-activation of c-Jun is abrogated by the serine 10-phosphorylated form of p27Kip1." dans: The Journal of biological chemistry, Vol. 277, Issue 36, pp. 32413-6, (2002) (PubMed).
Boehm, Yoshimoto, Crook, Nallamshetty, True, Nabel, Nabel: "A growth factor-dependent nuclear kinase phosphorylates p27(Kip1) and regulates cell cycle progression." dans: The EMBO journal, Vol. 21, Issue 13, pp. 3390-401, (2002) (PubMed).