Rabbit IgG polyclonal antibody for Bcl-2-binding component 3 (BBC3) detection. Tested with WB in Human, Mouse, Rat.
Immunogène
A synthetic peptide corresponding to a sequence at the C-terminal of human PUMA, identical to the related rat and mouse sequences.< br/>Immunogen was affinity purified.
At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.
Chipuk, Bouchier-Hayes, Kuwana, Newmeyer, Green: "PUMA couples the nuclear and cytoplasmic proapoptotic function of p53." dans: Science (New York, N.Y.), Vol. 309, Issue 5741, pp. 1732-5, (2005) (PubMed).
The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91 % amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.