ITK anticorps (AA 1-26)

N° du produit ABIN967287

Détail du produit anti-ITK anticorps

Antigène: ITK (IL2-Inducible T-Cell Kinase (ITK))

Épitope: AA 1-26

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ITK est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Fusion protein with aa.1-26 of human/mouse Itk

Clone: 2F12

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-ITK (ABIN967287)

Liu, Bunnell, Gurniak, Berg: "T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells." dans: The Journal of experimental medicine, Vol. 187, Issue 10, pp. 1721-7, (1998) (PubMed).

Heyeck, Wilcox, Bunnell, Berg: "Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity." dans: The Journal of biological chemistry, Vol. 272, Issue 40, pp. 25401-8, (1997) (PubMed).

Siliciano, Morrow, Desiderio: "itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 23, pp. 11194-8, (1993) (PubMed).

Détails sur ITK

Antigène: ITK (IL2-Inducible T-Cell Kinase (ITK))

Autre désignation: ITK (ITK Produits)

Sujet: Itk (IL-2 inducible tyrosine kinase) is a member of the TEC family of intracellular tyrosine kinases. Members of this family are characterized by their role in hematopoietic cell signaling. Itk is expressed primarily in T cells and has been shown to play a role in T-cell antigen receptor (TCR) mediated proliferation, interleukin-2 production, and T cell differentiation. Itk, following stimulation of the TCR becomes phosphorylated on tyrosine 511 by the Src kinase Lck resulting in increased in vitro Itk kinase activity. If phenylalanine is substituted for tyrosine at position 511, the in vivo kinase activity of Itk is drastically diminished, indicating that this site plays a key role in regulating Itk function. Itk migrates at ~72 kDa on SDS/PAGE. (SWISSPROT: Q08881)

Poids moléculaire: 72 kDa

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway