CD93 anticorps

N° du produit ABIN967346

Détail du produit anti-CD93 anticorps

Antigène: CD93

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD93 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: C1q-binding protein

Clone: R3

Isotype: IgM kappa

Information d'application

Indications d'application: Immunofluorescent Staining and Flow Cytometric Analysis:
The staining technique and blocking controls are described in detail by C. Prussin and D. Metcalfe. A suitable mouse IgM, kappa isotype control for assessing the level of background staining on human cells is recommended: use at comparable concentrations to antibody of interest (eg, Less or equal than 0.5 µg Ab/1 million cells) (see Figure).

Western Blotting and immunoprecipitation:
When run under non-reducing conditions, Human C1qRp migrates as a 100 kDa protein, due to high level of glycosylation C1qR migrates as 126 kDa under reducing conditions. The R3 antibody is suitable to detect C1qRp by Western blotting and immunprecipitation as described, however, the antibody is not tested for this application. Reactivity of the antibody with the reduced protein is dramatically decreased.

Modulation of monocyte phagocytic activity:
Pretreatment of monocytes with R3 neutralizes C1q-mediated enhancement of phagocytosis. In addition, when immobilized, R3 mimics C1q- MBL- or SPA-mediated enhancement of phagocytosis, however, the antibody is not tested for this application.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD93 (ABIN967346)

Danet, Luongo, Butler, Lu, Tenner, Simon, Bonnet: "C1qRp defines a new human stem cell population with hematopoietic and hepatic potential." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 16, pp. 10441-5, (2002) (PubMed).

Nepomuceno, Ruiz, Park, Tenner: "C1qRP is a heavily O-glycosylated cell surface protein involved in the regulation of phagocytic activity." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 6, pp. 3583-9, (1999) (PubMed).

Tenner: "C1q receptors: regulating specific functions of phagocytic cells." dans: Immunobiology, Vol. 199, Issue 2, pp. 250-64, (1999) (PubMed).

Nepomuceno, Tenner: "C1qRP, the C1q receptor that enhances phagocytosis, is detected specifically in human cells of myeloid lineage, endothelial cells, and platelets." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 4, pp. 1929-35, (1998) (PubMed).

Nepomuceno, Henschen-Edman, Burgess, Tenner: "cDNA cloning and primary structure analysis of C1qR(P), the human C1q/MBL/SPA receptor that mediates enhanced phagocytosis in vitro." dans: Immunity, Vol. 6, Issue 2, pp. 119-29, (1997) (PubMed).

Guan, Robinson, Goodman, Tenner: "Cell-surface protein identified on phagocytic cells modulates the C1q-mediated enhancement of phagocytosis." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 8, pp. 4005-16, (1994) (PubMed).

Guan, Burgess, Robinson, Goodman, McTigue, Tenner: "Phagocytic cell molecules that bind the collagen-like region of C1q. Involvement in the C1q-mediated enhancement of phagocytosis." dans: The Journal of biological chemistry, Vol. 266, Issue 30, pp. 20345-55, (1991) (PubMed).

Détails sur CD93

Antigène: CD93

Autre désignation: CDw93 (CD93 Produits)

Synonymes: anticorps 6030404G09Rik, anticorps AA145088, anticorps AA4.1, anticorps AW555904, anticorps C1qr1, anticorps C1qrp, anticorps Ly68, anticorps C1QR1, anticorps C1qR(P), anticorps C1qRP, anticorps CDw93, anticorps ECSM3, anticorps MXRA4, anticorps dJ737E23.1, anticorps C1qRp, anticorps CD93 antigen, anticorps CD93 molecule, anticorps Cd93, anticorps CD93

Sujet: The immunogen used to raise R3 was C1q-binding protein preparation as described. Human C1qRp is a 631 a.a. protein (~66.5 kD) protein that is highly expressed on monocytes/macrophages, neutrophil granulocytes but not on T and B lymphocytes. C1qRp binds C1q, the recognition subunit of the first component (C1) of the complement pathway, as well as MBL (Mannose-binding-lectin) and SPA (Pulmonary Surfactant Protein A). Human C1qRp is involved in the C1q-mediated enhancement of phagocytosis. R3 is suitable to detect C1qRp expression on cells of myeloid lineage by flow cytometry, C1qRp in cellular lysates by Western blotting or immunoprecipitation. Pretreatment of monocytes with R3 neutralizes C1q-mediated enhancement of phagocytosis. In addition, when immobilized, R3 mimics C1q- MBL- or SPA-mediated enhancement of phagocytosis as reported.
CDw93 (C1qRp) has been reported to define a human stem cell population with hematopoietic and hepatic potential.
Synonyms: C1qRp