1. Since applications vary, each investigator should titrate the reagent to obtain optimal results. 2. Please refer to us for technical protocols. 3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. 4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunohistochemistry: The E5 antibody, specific for human clusterin, is tested for immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed and formalin-fixed, paraffin-embedded sections. Tissue tested was human tonsil. The antibody stains dendritic cells in the follicle and basement membrane of epithelial cells. The isotype control recommended for use with this antibody is purified mouse IgG1. For optimal indirect immunohistochemical staining, the E5 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-mouse Igs (multiple adsorbed) as the secondary antibody and Streptavidin-HRP together with the DAB Detection System. More conveniently, the Anti-Mouse Ig HRP Detection Kit, which contains the biotinylated secondary antibody, antibody diluent, Streptavidin-HRP and DAB substrate, can be used for staining.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C.
Wellmann, Thieblemont, Pittaluga, Sakai, Jaffe, Siebert, Raffeld: "Detection of differentially expressed genes in lymphomas using cDNA arrays: identification of clusterin as a new diagnostic marker for anaplastic large-cell lymphomas." dans: Blood, Vol. 96, Issue 2, pp. 398-404, (2000) (PubMed).
Rosenberg, Silkensen: "Clusterin: physiologic and pathophysiologic considerations." dans: The international journal of biochemistry & cell biology, Vol. 27, Issue 7, pp. 633-45, (1995) (PubMed).
Murphy, Kirszbaum, Walker, dApice: "SP-40,40, a newly identified normal human serum protein found in the SC5b-9 complex of complement and in the immune deposits in glomerulonephritis." dans: The Journal of clinical investigation, Vol. 81, Issue 6, pp. 1858-64, (1988) (PubMed).
The E5 antibody reacts with human clusterin. Clusterin was originally isolated and characterized from glomerular immune deposits of patients with renal glomerulonephritis. Clusterin, an 80 kD disulfide-linked, heterodimeric glycoprotein (previously named SP-40,40 [serum protein 40 kD,40kD]) was shown to be part of the SC5b-9 complex of complement, yet separate and distinct from the S protein. Clusterins have since been identified in a number of mammalian species and have been implicated in diverse cellular activities, however, the exact role remains to be elucidated.