Cyclin B1 anticorps (AA 1-21)

N° du produit ABIN967426

Détail du produit anti-Cyclin B1 anticorps

Antigène: Cyclin B1 (CCNB1)

Épitope: AA 1-21

Reactivité: Humain, Souris, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin B1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), BioImaging (BI), Fluorescence Microscopy (FM)

Marque: BD Pharmingen™

Réactivité croisée: Hamster, Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Triton is a trademark of the Dow Chemical Company.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Cyclin B1 Recombinant Protein

Clone: GNS-1

Isotype: IgG1

Information d'application

Indications d'application: Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 min at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hr at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in dark for 1 hr at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 min before imaging.
11. View and analyze the cells on an appropriate imaging instrument.

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-Cyclin B1 (ABIN967426)

Gong, Traganos, Darzynkiewicz: "Expression of cyclins-B and cyclins-e in individual molt-4 cells and in stimulated human-lymphocytes during their progression through the cell-cycle." dans: International journal of oncology, Vol. 3, Issue 6, pp. 1037-42, (2011) (PubMed).

Darzynkiewicz, Gong, Juan, Ardelt, Traganos: "Cytometry of cyclin proteins." dans: Cytometry, Vol. 25, Issue 1, pp. 1-13, (1997) (PubMed).

Gong, Traganos, Darzynkiewicz: "Discrimination of G2 and mitotic cells by flow cytometry based on different expression of cyclins A and B1." dans: Experimental cell research, Vol. 220, Issue 1, pp. 226-31, (1995) (PubMed).

Gong, Ardelt, Traganos, Darzynkiewicz: "Unscheduled expression of cyclin B1 and cyclin E in several leukemic and solid tumor cell lines." dans: Cancer research, Vol. 54, Issue 16, pp. 4285-8, (1994) (PubMed).

Sherwood, Rush, Kung, Schimke: "Cyclin B1 expression in HeLa S3 cells studied by flow cytometry." dans: Experimental cell research, Vol. 211, Issue 2, pp. 275-81, (1994) (PubMed).

Zhang, Xiong, Beach: "Proliferating cell nuclear antigen and p21 are components of multiple cell cycle kinase complexes." dans: Molecular biology of the cell, Vol. 4, Issue 9, pp. 897-906, (1994) (PubMed).

Faha, Harlow, Lees: "The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2." dans: Journal of virology, Vol. 67, Issue 5, pp. 2456-65, (1993) (PubMed).

Coleman, Tang, Dunphy: "Negative regulation of the wee1 protein kinase by direct action of the nim1/cdr1 mitotic inducer." dans: Cell, Vol. 72, Issue 6, pp. 919-29, (1993) (PubMed).

Gong, Traganos, Darzynkiewicz: "Simultaneous analysis of cell cycle kinetics at two different DNA ploidy levels based on DNA content and cyclin B measurements." dans: Cancer research, Vol. 53, Issue 21, pp. 5096-9, (1993) (PubMed).

Kung, Sherwood, Schimke: "Differences in the regulation of protein synthesis, cyclin B accumulation, and cellular growth in response to the inhibition of DNA synthesis in Chinese hamster ovary and HeLa S3 cells." dans: The Journal of biological chemistry, Vol. 268, Issue 31, pp. 23072-80, (1993) (PubMed).

Sherwood, Kung, Roitelman, Simoni, Schimke: "In vivo inhibition of cyclin B degradation and induction of cell-cycle arrest in mammalian cells by the neutral cysteine protease inhibitor N-acetylleucylleucylnorleucinal." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3353-7, (1993) (PubMed).

Cao, Faha, Dembski, Tsai, Harlow, Dyson: "Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F." dans: Nature, Vol. 355, Issue 6356, pp. 176-9, (1992) (PubMed).

Faha, Ewen, Tsai, Livingston, Harlow: "Interaction between human cyclin A and adenovirus E1A-associated p107 protein." dans: Science (New York, N.Y.), Vol. 255, Issue 5040, pp. 87-90, (1992) (PubMed).

Détails sur Cyclin B1

Antigène: Cyclin B1 (CCNB1)

Autre désignation: Cyclin B1 (CCNB1 Produits)

Synonymes: anticorps ccnb, anticorps cycb, anticorps cb267, anticorps cycb1, anticorps wu:fa19g04, anticorps wu:fb16d01, anticorps wu:fb16e07, anticorps wu:fi21c01, anticorps ccnb1, anticorps MGC53596, anticorps CCNB, anticorps Ccnb1-rs1, anticorps Ccnb1-rs13, anticorps CycB1, anticorps Cycb-4, anticorps Cycb-5, anticorps Cycb1-rs1, anticorps cyclin B1, anticorps cyclin B1 S homeolog, anticorps ccnb1, anticorps ccnb1.2, anticorps ccnb1.S, anticorps CCNB1, anticorps Ccnb1, anticorps ccnb1.2.S

Sujet: Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catlytic subunits) to form complexes that regulate the progression of the cell cycle. The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins, such as p21 and p27 [Kip1]. Cyclin B1 is a mitotic cyclin, where expression is normally low in G0/G1, increases in S and is maximal during the G2/M phase. Cyclin B1 is rapidly degraded at the end of mitosis, and is required for cells to exit from mitosis. This antibody has been reported to react to hamster and mouse cyclin B1. In addition, the GNS-1 antibody has been reported to recognize an epitope between amino acids 1-21 of human cyclin B1.

Poids moléculaire: 62 kDa

Pathways: Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase