Nestin anticorps

N° du produit ABIN967492

Détail du produit anti-Nestin anticorps

Antigène: Nestin (NES)

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nestin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat (E15) spinal cord extracts

Clone: Rat 401

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-Nestin (ABIN967492)

Coppola, Dautréaux, Nomaksteinsky, Brunet: "Phox2b expression in the taste centers of fish." dans: The Journal of comparative neurology, Vol. 520, Issue 16, pp. 3633-49, (2013) (PubMed).

Tseng, Gruzdeva, Li, Chuang, Sung: "Identification of the Tctex-1 regulatory element that directs expression to neural stem/progenitor cells in developing and adult brain." dans: The Journal of comparative neurology, Vol. 518, Issue 16, pp. 3327-42, (2010) (PubMed).

Naritsuka, Sakai, Hashikawa, Mori, Yamaguchi: "Perisomatic-targeting granule cells in the mouse olfactory bulb." dans: The Journal of comparative neurology, Vol. 515, Issue 4, pp. 409-26, (2009) (PubMed).

Tran, Banisadr, Ren, Chenn, Miller: "Chemokine receptor expression by neural progenitor cells in neurogenic regions of mouse brain." dans: The Journal of comparative neurology, Vol. 500, Issue 6, pp. 1007-33, (2007) (PubMed).

Pecchi, Dallaporta, Charrier, Pio, Jean, Moyse, Troadec: "Glial fibrillary acidic protein (GFAP)-positive radial-like cells are present in the vicinity of proliferative progenitors in the nucleus tractus solitarius of adult rat." dans: The Journal of comparative neurology, Vol. 501, Issue 3, pp. 353-68, (2007) (PubMed).

Horky, Galimi, Gage, Horner: "Fate of endogenous stem/progenitor cells following spinal cord injury." dans: The Journal of comparative neurology, Vol. 498, Issue 4, pp. 525-38, (2006) (PubMed).

Sadgrove, Laskowski, Gray et al.: "Examination of granule layer cell count, cell density, and single-pulse BrdU incorporation in rat organotypic hippocampal slice cultures with respect to culture medium, septotemporal position, and ..." dans: The Journal of comparative neurology, Vol. 497, Issue 3, pp. 397-415, (2006) (PubMed).

Dahlstrand, Zimmerman, McKay, Lendahl: "Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments." dans: Journal of cell science, Vol. 103 ( Pt 2), Issue 1, pp. 589-97, (1993) (PubMed).

Tohyama, Lee, Rorke, Marvin, McKay, Trojanowski: "Monoclonal antibodies to a rat nestin fusion protein recognize a 220-kDa polypeptide in subsets of fetal and adult human central nervous system neurons and in primitive neuroectodermal tumor cells." dans: The American journal of pathology, Vol. 143, Issue 1, pp. 258-68, (1993) (PubMed).

Dahlstrand, Collins, Lendahl: "Expression of the class VI intermediate filament nestin in human central nervous system tumors." dans: Cancer research, Vol. 52, Issue 19, pp. 5334-41, (1992) (PubMed).

Tohyama, Lee, Rorke, Marvin, McKay, Trojanowski: "Nestin expression in embryonic human neuroepithelium and in human neuroepithelial tumor cells." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 66, Issue 3, pp. 303-13, (1992) (PubMed).

Renfranz, Cunningham, McKay: "Region-specific differentiation of the hippocampal stem cell line HiB5 upon implantation into the developing mammalian brain." dans: Cell, Vol. 66, Issue 4, pp. 713-29, (1991) (PubMed).

Lendahl, Zimmerman, McKay: "CNS stem cells express a new class of intermediate filament protein." dans: Cell, Vol. 60, Issue 4, pp. 585-95, (1990) (PubMed).

Geschwind, Hockfield: "Identification of proteins that are developmentally regulated during early cerebral corticogenesis in the rat." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 9, Issue 12, pp. 4303-17, (1990) (PubMed).

Frederiksen, McKay: "Proliferation and differentiation of rat neuroepithelial precursor cells in vivo." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 4, pp. 1144-51, (1988) (PubMed).

Hockfield, McKay: "Identification of major cell classes in the developing mammalian nervous system." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 5, Issue 12, pp. 3310-28, (1986) (PubMed).

Détails sur Nestin

Antigène: Nestin (NES)

Autre désignation: Nestin (NES Produits)

Synonymes: anticorps paranemin, anticorps NES, anticorps AA166324, anticorps C78523, anticorps ESTM46, anticorps nestin, anticorps nestin S homeolog, anticorps NES, anticorps nes.S, anticorps Nes, anticorps nes

Sujet: Multipotential stem cells in the neural tube of the developing embryo give rise to the different neuronal cell types of the brain. Nestin is an intermediate filament protein which is abundantly expressed in neuroepithelial stem cells early in embryogenesis, but is absent from nearly all mature central nervous system (CNS) cells. After its down-regulation, GFAP and neurofilaments are expressed in differentiated astrocytes and neurons, respectively. Initial studies showed that the mouse anti-rat nestin antibody (clone Rat 401) recognized transient radial glial cells and dividing neuroepithelial stem cells in the embryonic rat CNS. Reportedly, Rat 401 has been used to analyze nestin expression in the developing rat nervous system and in immortalized CNS precursor cell lines (e.g such as in E11 rat CNS stem cells, but lost by postnatal day 6 (P6) in spinal cord and by P21 in the cerebellum). Although not expressed in normal adult CNS cells, nestin has been reportedly to be detectable in a variety of CNS tumors, suggesting that these tumors share gene expression patterns with primitive, undifferentiated CNS cells. Rat 401 positive cells have been reported to be found throughout the developing neural tube, but not in the adult CNS. More than 90% of dissociated E11 neural tube cells have been reported to be Rat 401 positive. Rat 401 recognizes rat nestin and has been reported not to cross-react with human nestin. Due to differential tissue expression, this antibody may recognize nestin as a doublet within a range of 198-260 kD.

Poids moléculaire: 198-260 kDa