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CAN anticorps (AA 349-505)

CAN Reactivité: Humain WB, IP Hôte: Souris Monoclonal G182-1847 unconjugated
N° du produit ABIN967510
  • Antigène Voir toutes CAN Anticorps
    CAN (Calcineurin (CAN))
    Épitope
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 349-505
    Reactivité
    • 11
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 10
    • 2
    • 1
    Souris
    Clonalité
    • 10
    • 3
    Monoclonal
    Conjugué
    • 13
    Cet anticorp CAN est non-conjugé
    Application
    • 13
    • 10
    • 8
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP)
    Marque
    BD Pharmingen™
     Réactivité croisée
    Humain
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Human beta1 isoform of calcineurin A Subunit aa. 349-505
    Clone
    G182-1847
    Isotype
    IgG1
    Top Product
    Discover our top product CAN Anticorps primaire
  • Indications d'application
    Applications include immunoprecipitation (1-2 µg/ml) and western blot analysis (1-2 µg/ml). Jurkat human T cells (ATCC TIB-152)2, HeLa human carcinoma cells (ATCC CCL-2), and A-431 human epidermal carcinoma cells (ATCC CRL-1555) are suggested as positive controls. A431 cell lysate (ABIN968533) and HeLa cell lysate (ABIN968535) are also available as ready-to-use positive western blot control.
    Commentaires

    Related Products: ABIN967389, ABIN968533, ABIN968535

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C.
  • Lai, Burnett, Wolosker, Blackshaw, Snyder: "Cain, a novel physiologic protein inhibitor of calcineurin." dans: The Journal of biological chemistry, Vol. 273, Issue 29, pp. 18325-31, (1998) (PubMed).

    Husi, Luyten, Zurini: "Mapping of the immunophilin-immunosuppressant site of interaction on calcineurin." dans: The Journal of biological chemistry, Vol. 269, Issue 19, pp. 14199-204, (1994) (PubMed).

    Fruman, Klee, Bierer, Burakoff: "Calcineurin phosphatase activity in T lymphocytes is inhibited by FK 506 and cyclosporin A." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 9, pp. 3686-90, (1992) (PubMed).

  • Antigène
    CAN (Calcineurin (CAN))
    Autre désignation
    Calcineurin (CAN Produits)
    Synonymes
    anticorps CALN, anticorps CALNA, anticorps CALNA1, anticorps CCN1, anticorps CNA1, anticorps PPP2B, anticorps CAN A1/PP2B, anticorps CG1455, anticorps Can, anticorps Cana1, anticorps CnA, anticorps CnnA21EF, anticorps D14, anticorps DmCanA1-100B, anticorps Dmel\\CG1455, anticorps PP2B, anticorps PP2B 21EF, anticorps PpD14, anticorps canA, anticorps pMY4, anticorps 2900074D19Rik, anticorps AI841391, anticorps AW413465, anticorps CN, anticorps Caln, anticorps Calna, anticorps Calna1, anticorps protein phosphatase 3 catalytic subunit alpha, anticorps Calcineurin A1, anticorps protein phosphatase 3, catalytic subunit, alpha isoform, anticorps PPP3CA, anticorps CanA1, anticorps Ppp3ca
    Sujet
    Calcineurin is a Ca2+- and calmodulin-dependent serine/threonine phosphatase which consists of two subunits. The A subunit (~61 kDa) is catalytic and has calmodulinbinding activity, the B subunit (~19 kDa), which is myristylated at its N-terminus, has Ca[2+]-binding activity. Calcineurin is expressed ubiquitously in eukaryotic cells, including yeast. The protein is highly expressed in mammalian brain, and has been implicated in a number of calcium-dependent pathways in the nervous system, including regulation of neurotransmitter release. Calcineurin also plays a key role in T cell receptor-mediated signal transduction pathways. Calcineurin dephosphorylates components of NF-AT (Nuclear Factor of Activated T cells), allowing NF-AT to translocate to the nucleus where it participates with AP-1 in activation of multiple cytokine and surface receptor genes. Inhibition of calcineurin by immunosuppressive agents such as cyclosporin A and FK506 correlates with the suppression of early T cell activation events. These immunosuppressive agents bind to specific intracellular proteins, called immunophilins, to form complexes that inhibit calcineurin’s phosphatase activity. In the presence of these complexes, NF-AT remains phosphorylated and thus cannot translocate to the nucleus. A novel protein, Cain (calcineurin inhibitor), has been described which displays a similar pattern of neuronal and brain expression and has been shown to bind and inhibit calcineurin activity in vivo.
    G182-1847 recognizes the alpha and beta isoforms of the A subunit of calcineurin (~61 kDa). It reacts with an epitope in the autoinhibitory domain. It does not recognize the B subunit of calcineurin. A recombinant tag-fusion protein containing amino acids 349-505 of the human beta1 isoform of calcineurin A subunit was used as immunogen.
    Poids moléculaire
    61 kDa
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