P-Glycoprotein anticorps

N° du produit ABIN967562

Détail du produit anti-P-Glycoprotein anticorps

Antigène: P-Glycoprotein

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp P-Glycoprotein est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: 17F9

Isotype: IgG2b

Information d'application

Indications d'application: Positive control cells are available through special order

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-P-Glycoprotein (ABIN967562)

Shi, Wrin, Reeder, Liu, Ring: "High-affinity monoclonal antibodies against P-glycoprotein." dans: Clinical immunology and immunopathology, Vol. 76, Issue 1 Pt 1, pp. 44-51, (1995) (PubMed).

Bénard, Bourhis, Riou: "Clinical significance of multiple drug resistance in human cancers." dans: Anticancer research, Vol. 10, Issue 5A, pp. 1297-302, (1990) (PubMed).

Goldstein, Galski, Fojo, Willingham, Lai, Gazdar, Pirker, Green, Crist, Brodeur: "Expression of a multidrug resistance gene in human cancers." dans: Journal of the National Cancer Institute, Vol. 81, Issue 2, pp. 116-24, (1989) (PubMed).

Détails sur P-Glycoprotein

Antigène: P-Glycoprotein

Abstract: P-Glycoprotein Produits

Synonymes: anticorps ABC20, anticorps CD243, anticorps CLCS, anticorps GP170, anticorps MDR1, anticorps P-GP, anticorps PGY1, anticorps Abcb1, anticorps Mdr1a, anticorps ABCB1, anticorps ABCB4, anticorps CMDR1, anticorps xemdr, anticorps ATP binding cassette subfamily B member 1, anticorps ATP binding cassette subfamily B member 1A, anticorps ATP binding cassette subfamily B member 1 L homeolog, anticorps ABCB1, anticorps Abcb1a, anticorps abcb1.L

Sujet: Reacts with a 170-180 kDa transmembrane glycoprotein (P-glycoprotein), a product of the multidrug resistance-1 (MDR1) gene. This glycoprotein is expressed on MDR positive cells and has been reported to be expressed on many normal tissues, such as adrenal glands and endothelium, in the brain and skin. P-glycoprotein is known to impart drug resistance to cells by pumping many anti-cancer drugs out of the cytoplasm. 17F9 antibody binds to the surface of the viable, unfixed MDR+ cell line, PMG-Y (please inquire), and is able to partially block the binding of UIC2 antibody (another MDR-specific monoclonal antibody). Immunoprecipitation application is reported, but not routinely tested in house.
Synonyms: MDR