ANK2 anticorps

N° du produit ABIN967633

Détail du produit anti-ANK2 anticorps

Antigène: ANK2 (Ankyrin 2, Neuronal (ANK2))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ANK2 est non-conjugé

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Marque: BD Pharmingen™

Réactivité croisée: Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Ankyrin B Spectrin Binding Domain Peptide

Clone: 2-20

Isotype: IgG1

Information d'application

Indications d'application: Neonatal rat brain membranes are suggested for use as the positive control.

Commentaires:

Related Products: ABIN967389, ABIN968545, ABIN968546

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4° C.

Références pour anticorps anti-ANK2 (ABIN967633)

Scotland, Zhou, Benveniste, Bennett: "Nervous system defects of AnkyrinB (-/-) mice suggest functional overlap between the cell adhesion molecule L1 and 440-kD AnkyrinB in premyelinated axons." dans: The Journal of cell biology, Vol. 143, Issue 5, pp. 1305-15, (1999) (PubMed).

Kordeli, Lambert, Bennett: "AnkyrinG. A new ankyrin gene with neural-specific isoforms localized at the axonal initial segment and node of Ranvier." dans: The Journal of biological chemistry, Vol. 270, Issue 5, pp. 2352-9, (1995) (PubMed).

Bennett, Gilligan: "The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane." dans: Annual review of cell biology, Vol. 9, pp. 27-66, (1994) (PubMed).

Otto, Kunimoto, McLaughlin, Bennett: "Isolation and characterization of cDNAs encoding human brain ankyrins reveal a family of alternatively spliced genes." dans: The Journal of cell biology, Vol. 114, Issue 2, pp. 241-53, (1991) (PubMed).

Kordeli, Davis, Trapp, Bennett: "An isoform of ankyrin is localized at nodes of Ranvier in myelinated axons of central and peripheral nerves." dans: The Journal of cell biology, Vol. 110, Issue 4, pp. 1341-52, (1990) (PubMed).

Détails sur ANK2

Antigène: ANK2 (Ankyrin 2, Neuronal (ANK2))

Autre désignation: Ankyrin B (ANK2 Produits)

Synonymes: anticorps ANK-2, anticorps LQT4, anticorps brank-2, anticorps AI835472, anticorps AW491075, anticorps Ank-2, anticorps Ank3, anticorps CHANK2, anticorps ANK2, anticorps ank2, anticorps zgc:101738, anticorps ANKB, anticorps ankyrin 2, anticorps ankyrin 2, brain, anticorps ankyrin-2, anticorps ankyrin 2b, neuronal, anticorps ANK2, anticorps Ank2, anticorps LOC539238, anticorps ank2b, anticorps LOC100465763

Sujet: Ankyrins are a family of proteins which were first discovered in erythrocytes and shown to bind to spectrin, a primary component of the cytoskeletal architecture. They have since been shown to be expressed at very high levels in the vertebrate brain and are also found in other cell types. Ankyrins function by acting as adaptors to link integral membrane proteins, including cell adhesion and ion channel molecules to the cytoskeleton. The structure of ankyrin consists of three domains, two highly conserved N-terminal and one C-terminal variable domain. The conserved regions contain a membrane-binding domain of ~89-95 kDa and a spectrin-binding domain of ~62 kDa, while the variable region has pre-mRNA alternative splice sites. Two ankyrins have been extensively characterized: ankryin R is the product of the ANK1 gene and is primarily expressed in the brain, although alternatively spliced forms are found in erythrocytes, ankyrin B is the product of the ANK2 gene, located on a different chromosome from ANK1, and is primarly expressed in neonatal and adult brain but is also found in heart, lymphocytes and platelets. Studies done on ankyrinB (-/-) knockout mice, which die by postnatal day 21, have demonstrated a link between the L1 cell adhesion molecule and ankyrin B in premyelinated axons. Isoforms of ankyrin B have been reported to be detectable ranging from approximately 440, 220, 150 and 110 kDa in western blot analysis, depending on the tissue-type of origin. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 110-440 kDa

Pathways: Cell-Cell Junction Organization